Yang Wei, Chen Quanyu, Bai Lianhua
Hepatobiliary Institute, Southwest Hospital, Army Medical University, No. 30 GaotanYan, ShapingBa District, Chongqing 400038, China.
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China.
Data Brief. 2018 Jul 7;22:349-358. doi: 10.1016/j.dib.2018.07.021. eCollection 2019 Feb.
The data presented in this article are related to the original research article entitled "A novel bioscaffold with naturally-occurring extracellular matrix promotes hepatocyte survival and vessel patency in mouse models of heterologous transplantation" (Yang et al., in press) [1]. This article describes a decellularized liver scaffold (DLS) that derived from partial hepatectomy liver (rDLS) which supported primary hepatocyte survival and promoted blood patency, as compared with a conventional scaffold that generated from naïve liver (nDLS). Analysis by immunochemistry and scanning electron microscope (SEM) showed that the vessel network and extracellular matrix (ECM) components were similar to the nDLS. The rDLS could prevent blood clotting after transplanted it in vivo, identified by immunofluorescence staining for the integrin (αIIb, α4) expression and liver transplantation models (mice, pigs) a formed well-blood petency liver lobules. These data indicate that the novel scaffold (rDLS) with naturally-occurring "activated ECM" that may be useful for the implantation in vivo of a bioengineered organoid that is able to exert function long term without clotting in future clinic.
本文所呈现的数据与原创研究文章《一种具有天然细胞外基质的新型生物支架促进异源移植小鼠模型中肝细胞存活和血管通畅》（杨等人，即将发表）[1]相关。本文描述了一种源自部分肝切除肝脏的去细胞肝脏支架（DLS，rDLS），与由未处理肝脏生成的传统支架（nDLS）相比，它能支持原代肝细胞存活并促进血液通畅。免疫化学和扫描电子显微镜（SEM）分析表明，血管网络和细胞外基质（ECM）成分与nDLS相似。通过整合素（αIIb、α4）表达的免疫荧光染色以及肝移植模型（小鼠、猪）证实，rDLS在体内移植后可防止血液凝固，形成了血液通畅良好的肝小叶。这些数据表明，具有天然“活化ECM”的新型支架（rDLS）可能有助于在未来临床中对能够长期发挥功能且不发生凝血的生物工程类器官进行体内植入。
