Koga S, Kiyohara T, Taniguchi K, Nishikido M, Kubota S, Sakuragi T, Shindo K, Saitoh Y
Department of Urology, Nagasaki University School of Medicine, Japan.
Urol Res. 1988;16(5):351-5. doi: 10.1007/BF00256041.
To investigate the mechanism of Bacillus de Calmette Guérin (BCG) bladder instillation therapy, the killer cell activity induced in peripheral blood mononuclear cells (PBMNCs) after BCG instillation was examined. Significant cytotoxic activity against natural killer (NK) cell resistant target tumor cells was detected after 3 days of instillation. To characterize this BCG induced cytotoxic activity further, human PBMNCs were cultured with BCG in vitro. From 24 h maximum cytotoxicity was obtained and continued for 3 days, then decreased slightly. Neither a DNA synthesis inhibitor Cytosine-arabinoside (Ara-C) nor a cytotoxic T cell (CTL) generation inhibitor Cyclosporine A inhibited this killer cell activation. Monoclonal antibody treatment revealed that both precursor and effector cells are Leu1-, 3a-, 7+, 11b+. The recognition specificity from cold target competition experiments was selective. Taken together NK type precursor was activated with BCG into NK type effector which has wider spectrum of target cells than usual NK cell.
为了研究卡介苗（BCG）膀胱灌注治疗的机制，检测了卡介苗灌注后外周血单个核细胞（PBMNCs）诱导的杀伤细胞活性。灌注3天后检测到对自然杀伤（NK）细胞抗性靶肿瘤细胞的显著细胞毒性活性。为了进一步表征这种卡介苗诱导的细胞毒性活性，将人PBMNCs与卡介苗在体外培养。从24小时开始获得最大细胞毒性，并持续3天，然后略有下降。DNA合成抑制剂阿糖胞苷（Ara-C）和细胞毒性T细胞（CTL）生成抑制剂环孢素A均未抑制这种杀伤细胞的激活。单克隆抗体治疗显示前体细胞和效应细胞均为Leu1-、3a-、7+、11b+。冷靶竞争实验的识别特异性具有选择性。综上所述，NK型前体细胞被卡介苗激活为NK型效应细胞，其靶细胞谱比通常的NK细胞更宽。
