Environmental Research Laboratory, P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu, India.
Environmental Research Laboratory, P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu, India.
Environ Toxicol Pharmacol. 2019 Feb;66:55-61. doi: 10.1016/j.etap.2018.12.011. Epub 2018 Dec 19.
The present study was aimed to analyze the effect of ZnO and TiO nanoparticles (NPs) on Haemato-immunological parameters in adult Tilapia, Oreochromis mossambicus. The nanoparticles size found as 47 nm and 30 nm for ZnO and TiO respectively. The acute toxicity (96 h) of ZnO (LC50: 100-110 ppm) and TiO (LC50: 80-90 ppm) NPs were identified by using probit analysis. RBC, Hb and HCT levels decreased in nanoparticles exposed groups resulted in decreased oxygen carrying capacity of RBC and other erythrocyte indices (MCH, MCV, MCHC). Increased WBC, neutrophils & monocytes and decreased lymphocyte levels were observed as increased concentration of the nanoparticles. The results were found as statistically significant (p < 0.05). In conclusion, the present study depicts that ZnO NPs exhibits more toxicity than TiO NPs. Nanoparticles presence even in low concentration (ppm) cause damage to the connective tissues of fish, so the existing permissible levels of these nanoparticles in water are need to be revised.
本研究旨在分析 ZnO 和 TiO 纳米粒子（NPs）对成年罗非鱼（Oreochromis mossambicus）血液免疫参数的影响。发现 ZnO 和 TiO 的纳米粒子大小分别为 47nm 和 30nm。通过概率分析确定了 ZnO（LC50：100-110ppm）和 TiO（LC50：80-90ppm）NPs 的急性毒性（96h）。暴露于纳米粒子的 RBC、Hb 和 HCT 水平下降，导致 RBC 和其他红细胞指标（MCH、MCV、MCHC）的携氧能力下降。随着纳米粒子浓度的增加，观察到白细胞、中性粒细胞和单核细胞增加，淋巴细胞减少。结果具有统计学意义（p<0.05）。总之，本研究表明 ZnO NPs 比 TiO NPs 表现出更高的毒性。即使在低浓度（ppm）下存在纳米粒子也会对鱼类的结缔组织造成损害，因此需要修订水中这些纳米粒子的现有允许水平。
