多药耐药表型：红白血病细胞表型诱导与其特性的关系。
Multidrug resistance phenotype: Relation between phenotype induction and its characteristics in erythroleukemia cells.
机构信息
Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande- FURG, Av. Itália, Km 8, CEP 96203-900 Rio Grande, RS, Brasil.
Laboratório de Cultura de Celular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG, Av. Itália, Km 8, CEP 96203-900 Rio Grande, RS, Brasil.
出版信息
Cell Biol Int. 2019 Feb;43(2):214-219. doi: 10.1002/cbin.11095.
Chemotherapy may be followed by multiple drug resistance (MDR). This is an obstacle in the treatment of cancer. It is therefore essential to understand the mechanisms underlying tumor resistance, especially those involved in the cell target/MDR relationship. To investigate this, the effects of exposing cells to UVB (to target DNA), UVA, and H O (to target the cell membrane) were observed in K562 (non MDR) and FEPS (MDR) cell lines. The K562 cells were more sensitive to UVA than the FEPS cells. The FEPS cell line was more resistant to H O than K562, only presenting cytotoxicity 72 h after being exposed to 40 mM, with no ROS increase until 48 h. Both cell lines were sensitive to UVB, presenting cytotoxicity after 24 h, mainly by apoptosis, and showed an increase in ROS levels. Our results indicate that agents acting on DNA may be able to overcome the MDR phenotype.
化疗后可能会出现多药耐药（MDR）。这是癌症治疗中的一个障碍。因此，了解肿瘤耐药的机制至关重要，特别是涉及细胞靶点/MDR 关系的机制。为了研究这一点，观察了 UVB（针对 DNA）、UVA 和 H₂O₂（针对细胞膜）暴露对 K562（非 MDR）和 FEPS（MDR）细胞系的影响。与 FEPS 细胞相比，K562 细胞对 UVA 更敏感。与 K562 相比，FEPS 细胞系对 H₂O₂的抵抗力更强，仅在暴露于 40mM 48 小时后才表现出细胞毒性，并且直到 48 小时才出现 ROS 增加。两种细胞系对 UVB 均敏感，在 24 小时后表现出细胞毒性，主要通过凋亡，并且 ROS 水平升高。我们的结果表明，作用于 DNA 的药物可能能够克服 MDR 表型。
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