Fernandes E Silva E, Figueira F S, Lettnin A P, Salgado M T S F, Lopes A C, Rehbein F, Kalil S J, Votto A P S
Laboratório de Cultura Celular, Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil.
Programa de Pós-Graduação em Ciências Fisiológicas, ICB, FURG, Rio Grande, RS, Brazil.
Mol Biol Rep. 2019 Feb;46(1):1349-1356. doi: 10.1007/s11033-018-4569-x. Epub 2018 Dec 15.
In this work we investigated the possible cellular changes induced by C-phycocyanin (C-PC) in erythroleukemic cells with (K562-Lucena and FEPS) and without (K562) the multidrug resistance (MDR) phenotype. The reactive oxygen levels (ROS) (evaluated by fluorimetry) were increased relative to the control in K562 and K562-Lucena cells treated with 100 µg/mL and were not increased in FEPS cells. The expression of the following genes related to resistance was evaluated by real-time PCR: COX2, ALOX5, ABCB1 and ABCC1. Treatment with 100 µg/mL of C-PC increased COX2 and ABCB1 expression in K562-Lucena cells and reduced expression of ALOX5 in K562-Lucena and FEPS cells. ROS levels appear to be involved in biological responses to C-PC in K562 and K562-Lucena cells, although expression of the genes studied here was only modified by C-PC in the K562-Lucena cell line. Thus, it is possible to suggest that C-PC modulates the expression of COX2 and ABCB1 for the K562-Lucena in a ROS-dependent manner and the expression of ALOX5 for the FEPS in a ROS-independent manner; however, more studies are needed to elucidate these mechanisms.
在这项研究中，我们调查了藻蓝蛋白（C-PC）对具有（K562-Lucena和FEPS）和不具有（K562）多药耐药（MDR）表型的红白血病细胞可能诱导的细胞变化。用荧光法评估的活性氧水平（ROS）在100μg/mL C-PC处理的K562和K562-Lucena细胞中相对于对照有所增加，而在FEPS细胞中未增加。通过实时PCR评估了以下与耐药相关基因的表达：COX2、ALOX5、ABCB1和ABCC1。用100μg/mL C-PC处理可增加K562-Lucena细胞中COX2和ABCB1的表达，并降低K562-Lucena和FEPS细胞中ALOX5的表达。ROS水平似乎参与了K562和K562-Lucena细胞对C-PC的生物学反应，尽管此处研究的基因表达仅在K562-Lucena细胞系中被C-PC改变。因此，有可能表明C-PC以ROS依赖的方式调节K562-Lucena细胞中COX2和ABCB1的表达，并以ROS独立的方式调节FEPS细胞中ALOX5的表达；然而，需要更多的研究来阐明这些机制。
