Department of Science, Waterford Institute of Technology, Waterford, Ireland.
Molecular Biotechnology and Biocatalysis Group, Pharmaceutical and Molecular Biotechnology Research Center, Waterford Institute of Technology, Waterford, Ireland.
Microbiologyopen. 2019 Apr;8(4):e00700. doi: 10.1002/mbo3.700. Epub 2018 Dec 30.
Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade characterizations.
腈酶（EC 3.5.5.1）负责将腈直接水合为相应的羧酸和氨。在生物制药和工业应用的生物催化中使用腈酶有助于向绿色化学转变。本研究提出了一种新的、特定分支的降落 PCR 检测新的腈酶基因的方法。该研究在 7 个属中鉴定了 15 个新的腈酶基因的部分序列，在另外 16 个属中显示了部分 DNA 序列同源性（%）。这项研究将在筛选微生物以鉴定新的特定分支的腈酶基因方面具有重要价值，根据其分支特征预测其对映选择性谱。
