毛细管区带电泳同时检测反油酸和顺-11-十八碳烯酸反式脂肪酸异构体的筛查方法
Screening method for simultaneous detection of elaidic and vaccenic trans fatty acid isomers by capillary zone electrophoresis.
作者信息
Amorim Tatiane Lima, Duarte Lucas Mattos, Dos Santos Hélio F, de Oliveira Marcone Augusto Leal
机构信息
Grupo de Química Analítica e Quimiometria - GQAQ, Department of Chemistry, Institute of Exact Sciences, Federal University of Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil.
Núcleo de Estudos Em Química Computacional - NEQC, Department of Chemistry, Institute of Exact Sciences, Federal University of Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil.
出版信息
Anal Chim Acta. 2019 Feb 7;1048:212-220. doi: 10.1016/j.aca.2018.10.057. Epub 2018 Oct 26.
A novel screening method for the simultaneous detection of elaidic and vaccenic trans fatty acid isomers by CZE-UV was proposed and applied to brazilian spreadable cheese analysis. Optimized background electrolyte was composed by 10 mmol L heptakis (2, 3, 6-tri-o-methyl)-beta cyclodextrin, 24 mmol L sodium tetraborate buffer solution, 45% methanol and 15% acetonitrile. Sample preparation comprised only a saponification reaction and dilution of sample solution before analysis. The method proved to be simple, environmental friendly and sensitive (LOD calculated for elaidic and vaccenic acid were 0.0163 mmol L and 0.0169 mmol L, respectively) and may be used to detect adulteration in dairy products, where the addition of hydrogenated vegetable fat to food is prohibited.
提出了一种采用毛细管区带电泳-紫外检测法同时检测反油酸和顺-11-十八碳烯酸反式脂肪酸异构体的新型筛选方法，并将其应用于巴西涂抹奶酪的分析。优化后的背景电解质由10 mmol/L七（2,3,6-三-O-甲基）-β-环糊精、24 mmol/L四硼酸钠缓冲溶液、45%甲醇和15%乙腈组成。样品制备仅包括皂化反应和分析前样品溶液的稀释。该方法被证明简单、环保且灵敏（反油酸和顺-11-十八碳烯酸的计算检测限分别为0.0163 mmol/L和0.0169 mmol/L），可用于检测乳制品中的掺假情况，在食品中禁止添加氢化植物油。
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