Muniswami Durai Murugan, Tharion George
Department of Physical Medicine and Rehabilitation, Christian Medical College, Vellore, Tamil Nadu, India.
Int J Appl Basic Med Res. 2018 Oct-Dec;8(4):220-226. doi: 10.4103/ijabmr.IJABMR_436_17.
Spinal cord injury (SCI) leads to permanent functional deficits because the central nervous system lacks the ability for spontaneous repair. Cell therapy strategies offered a hope in neurological repair. The clinical use of human embryonic stem cell transplantation is hampered by scientific and ethical controversies. Olfactory ensheathing cells (OECs)/bone marrow mesenchymal stem cell (MSC) is a promising cell source for autologous neurotransplantation devoid of ethical concerns.
This study aimed to evaluate the combined therapeutic effect of OEC, MSC, and chondroitinase in SCI rat models.
Adult female albino Wistar rats were divided into ten groups, = 6 rats in each group and control ( = 11). T10 level laminectomy was done in anesthetized rats to create drop-weight SCI. Both OEC and MSC were transplanted on the 9 day following SCI as a combined therapy with different dosage of 2 × 10, 5 × 10, 10 × 10, and >10 × 10 at a ratio of 1:1 with/without chondroitinase (0.2 U). One group of SCI rats was treated with chondroitinase alone 0.2 U. Dulbecco's Modified Eagle medium was injected in control rats. The outcome of transplantation was assessed using Basso, Beattie, Bresnahan (BBB) scale and motor-evoked potential studies.
All the treated groups showed hindlimb motor recovery in BBB score except control group ( < 0.05). All the three combinations showed better results than OEC + MSC groups in hindlimb motor recovery. In dose-response relationship, 5- and 10-lakh combinations elicited increased functional recovery than 2- and more than 10-lakh combinations. However, chondroitinase alone demonstrated a highest BBB score than any other groups.
Chondroitinase/cell combinations have a therapeutic beneficial effect in SCI.
脊髓损伤（SCI）会导致永久性功能缺陷，因为中枢神经系统缺乏自发修复能力。细胞治疗策略为神经修复带来了希望。人类胚胎干细胞移植的临床应用受到科学和伦理争议的阻碍。嗅鞘细胞（OECs）/骨髓间充质干细胞（MSC）是一种有前景的细胞来源，可用于自体神经移植，不存在伦理问题。
本研究旨在评估OEC、MSC和软骨素酶在SCI大鼠模型中的联合治疗效果。
成年雌性白化Wistar大鼠分为十组，每组6只大鼠，对照组11只。对麻醉的大鼠进行T10节段椎板切除术以造成重物打击性脊髓损伤。在脊髓损伤后第9天，将OEC和MSC作为联合治疗进行移植，不同剂量分别为2×10、5×10、10×10和>10×10，比例为1:1，同时使用/不使用软骨素酶（0.2 U）。一组脊髓损伤大鼠单独用0.2 U软骨素酶治疗。对照组大鼠注射杜尔贝科改良伊格尔培养基。使用巴索、比蒂、布雷斯纳汉（BBB）评分和运动诱发电位研究评估移植结果。
除对照组外，所有治疗组的BBB评分均显示后肢运动恢复（P<0.05）。在所有三种组合中，后肢运动恢复情况均优于OEC+MSC组。在剂量反应关系方面，50万和100万细胞组合比20万和超过100万细胞组合能引起更好的功能恢复。然而，单独使用软骨素酶的组显示出比其他任何组都更高的BBB评分。
软骨素酶/细胞组合在脊髓损伤中具有治疗有益效果。
