Department of Chemical Engineering, Faculty of Engineering, Istanbul University-Cerrahpasa, 34320, Avcılar, Istanbul, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcılar, Istanbul, Turkey.
Carbohydr Polym. 2019 Mar 1;207:580-587. doi: 10.1016/j.carbpol.2018.12.013. Epub 2018 Dec 10.
The aim of this study is to prepare the long linear aliphatic amine pendant group-functionalized chitosan based nanoparticulate gene carrier system with improved properties for the efficient transfection. The amine functionalized chitosan (MChi) was synthesized by using N-(2-hydroxyethyl)ethylenediamine (HE-EDA) and characterized for the first time. The nanoparticles of MChi (nMChi) were prepared by ionic gelation method, and their particle size, polydispersity (PDI), zeta potential (mV), gene binding capacity and cytotoxicity were determined. Green Fluorescent Protein circular plasmid DNA (pEGFN1) loaded nanoparticles (gnMChi) were used in the transfection studies. The results showed that nMChi with a particle size of 102.9 nm and zeta potential of 41.9 ± 5.63 mV was non-toxic, had high transfection efficiency in Human Embryonic Kidney 293 and Primary Ovine Fibroblast cell lines and would be used as an efficient gene carrier system.
本研究旨在制备具有改进性能的长线性脂肪族胺侧基功能化壳聚糖纳米粒基因载体系统，以实现高效转染。通过使用 N-(2-羟乙基)乙二胺（HE-EDA）首次合成了胺功能化壳聚糖（MChi）。采用离子凝胶化法制备了 MChi 的纳米颗粒（nMChi），并对其粒径、多分散性（PDI）、Zeta 电位（mV）、基因结合能力和细胞毒性进行了测定。绿色荧光蛋白圆形质粒 DNA（pEGFN1）负载的纳米颗粒（gnMChi）用于转染研究。结果表明，粒径为 102.9nm，Zeta 电位为 41.9±5.63mV 的 nMChi 无毒性，在人胚肾 293 细胞和原代绵羊成纤维细胞系中具有较高的转染效率，可作为高效的基因载体系统。
