Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
J Microbiol Immunol Infect. 2020 Aug;53(4):592-603. doi: 10.1016/j.jmii.2018.10.010. Epub 2018 Nov 17.
Angiostrongylus cantonensis is an important etiologic agent of eosinophilic meningitis and/or eosinophilic meningoencephalitis in humans. Th2 responses have been considered to be predominant in non-permissive hosts. However, changes of cytokines in the central nervous system of the host remain unclear. The present study was conducted to determine the temporal-spatial expressions of IL-4, IL-10, and IL-13 in the brains of infected C57BL/6 and BALB/c mice by immunohistochemistry.
After infecting each mouse with 25 third-stage larvae (L3), brain specimens were collected on day 7 and day 28 post-infection. Each specimen was cut into five sections and stained with corresponding antibodies of the three cytokines.
In infected C57BL/6 mice, high IL-4 expressions were found in the isocortex, IL-10 in the isocortex, olfactory area, hippocampus, cerebral nuclei, hypothalamus, cerebellum nuclei, and medulla, and IL-13 in the isocortex and cerebellum. In infected BALB/c mice, IL-4 and IL-10 were highly expressed in the isocortex, olfactory areas, cerebral nuclei, hypothalamus, and cerebellum nuclei and IL-13 in the thalamus and hypothalamus. High levels of the cytokines were usually detected in on day 7 in BALB/c mice and day 28 in C57BL/6 mice.
The special temporal-spatial expression changes of these three cytokines in the infected mouse brain may explain the differences in the survival and the time of occurrence of immune responses in the hosts after A. cantonensis infection.
广州管圆线虫是引起人体嗜酸性脑膜炎和/或嗜酸性脑膜脑炎的重要病原体。Th2 反应被认为在非允许宿主中占优势。然而，宿主中枢神经系统中细胞因子的变化尚不清楚。本研究通过免疫组织化学法检测感染 C57BL/6 和 BALB/c 小鼠大脑中 IL-4、IL-10 和 IL-13 的时空表达。
将 25 条第三期幼虫（L3）感染每只小鼠后，于感染后第 7 天和第 28 天采集脑标本。每个标本切成 5 个切片，用三种细胞因子的相应抗体染色。
在感染的 C57BL/6 小鼠中，高表达的 IL-4 见于大脑皮质，IL-10 见于大脑皮质、嗅区、海马、脑核、下丘脑、小脑核和延髓，IL-13 见于大脑皮质和小脑。在感染的 BALB/c 小鼠中，IL-4 和 IL-10 高表达于大脑皮质、嗅区、脑核、下丘脑和小脑核，IL-13 高表达于丘脑和下丘脑。在 BALB/c 小鼠中，这些细胞因子通常在感染后第 7 天达到高水平，而在 C57BL/6 小鼠中则在第 28 天达到高水平。
感染小鼠大脑中这三种细胞因子的特殊时空表达变化可能解释了 A. cantonensis 感染后宿主的存活和免疫反应发生时间的差异。
