Rukosuev V S, Milovanov A P, Fokin E I
Arkh Patol. 1988;50(9):17-22.
Distribution of the eight components of extracellular matrix in small villi of human placenta was studied with indirect immunofluorescence. Normal full-term pregnancy was found to present rough fibers and type I collagen conglomerates accumulated in the stromal center, while type III and V collagens occurred all over the villous surface. Stromal localization of type IV collagen was recorded as well as its presence in basal membranes of the epithelium and vessels. Fibronectin showed diffuse distribution concentrating along the vessels. Laminin, entactin and in lesser degree heparan sulphate proteoglycan were registered in epithelial and vascular basal membranes. Isolated sclerosis of the villi in pathological pregnancy involved stromal accumulation of type I, III collagens, small fragments of collagens type IV, V and fibronectin. Multiple sclerosis of adherent villi demonstrated hyalin-like conglomerates of various collagens and fibronectin filling up the stroma. Laminin, heparan sulphate proteoglycan and entactin were not observed at these sites.
采用间接免疫荧光法研究了人胎盘小绒毛细胞外基质八种成分的分布。结果发现，正常足月妊娠时，粗纤维和I型胶原聚集体积聚在基质中心，而III型和V型胶原则分布于绒毛表面。IV型胶原在基质中定位，同时也存在于上皮和血管的基底膜中。纤连蛋白呈弥漫性分布，沿血管集中。层粘连蛋白、巢蛋白以及程度较轻的硫酸乙酰肝素蛋白聚糖在上皮和血管基底膜中被检测到。病理妊娠时绒毛的局灶性硬化涉及I型、III型胶原，IV型、V型胶原小片段以及纤连蛋白在基质中的积聚。粘连绒毛的多发性硬化表现为各种胶原和纤连蛋白的透明样聚集体充满基质。在这些部位未观察到层粘连蛋白、硫酸乙酰肝素蛋白聚糖和巢蛋白。
