Mounier F, Foidart J M, Gubler M C
Lab Invest. 1986 Apr;54(4):394-401.
The distribution of types I, III, and IV collagens, fibronectin, laminin, and heparan sulfate proteoglycan during human fetal kidney development has been studied by indirect immunofluorescence. Fibronectin and interstitial collagens types I and III are present in undifferentiated mesenchyme, whereas the intrinsic basement membrane components, type IV collagen, laminin, and heparan sulfate proteoglycan are not detected. In differentiated cortex, types I and III collagens are detected only as interstitial connective fibers, whereas fibronectin is found in both interstitium and glomeruli where its distribution is dependent on the stage of maturation. It is found in both the mesangium and along capillary walls of immature glomeruli, and principally in the mesangium of mature ones. Basement membrane components delineated the branching ureteric bud. They also outlined the structures of the earliest stage of nephrogenesis (epithelial differentiation). In mature nephrons, they are found along glomerular, capsular, and tubular basement membranes.
通过间接免疫荧光法研究了人胎儿肾脏发育过程中I型、III型和IV型胶原蛋白、纤连蛋白、层粘连蛋白和硫酸乙酰肝素蛋白聚糖的分布情况。纤连蛋白以及I型和III型间质胶原蛋白存在于未分化的间充质中，而未检测到固有基底膜成分IV型胶原蛋白、层粘连蛋白和硫酸乙酰肝素蛋白聚糖。在分化的皮质中，I型和III型胶原蛋白仅作为间质结缔组织纤维被检测到，而纤连蛋白在间质和肾小球中均有发现，其分布取决于成熟阶段。在未成熟肾小球的系膜和毛细血管壁上均能发现纤连蛋白，而在成熟肾小球中主要存在于系膜中。基底膜成分勾勒出分支的输尿管芽。它们还勾勒出肾发生最早阶段（上皮分化）的结构。在成熟肾单位中，它们存在于肾小球、囊和肾小管的基底膜上。
