Ayrton A D, Trinick J, Wood B P, Smith J N, Ioannides C
Department of Biochemistry, University of Surrey, Guildford, U.K.
Biochem Pharmacol. 1988 Dec 1;37(23):4565-71. doi: 10.1016/0006-2952(88)90673-9.
The ability of the aza-aromatic polycyclic aromatic hydrocarbons 10-azobenz(a)pyrene and benz(a)acridine to induce the rat hepatic microsomal mixed-function oxidases was compared to that of their non-heterocyclic analogues benz(a)pyrene and benz(a)anthracene respectively. All four hydrocarbons markedly increased the O-deethylations of ethoxyresorufin and ethoxycoumarin, the non-heterocyclic analogues being the more potent. A more modest increase was seen in the O-dealkylation of pentoxyresorufin. All four hydrocarbons induced proteins recognised by antibodies to cytochrome P-450IAI but no increase was seen when antibodies to cytochrome P-450IIB1 were employed. The metabolic activation of benz(a)pyrene and Glu-P-1 to mutagenic intermediates in the Ames test was enhanced by all pretreatments. It is concluded that the aza-aromatic polycyclic hydrocarbons, like their non-heterocyclic analogues, selectively induce the cytochrome P-450I family of proteins.
将氮杂芳族多环芳烃10-氮杂苯并(a)芘和苯并(a)吖啶诱导大鼠肝微粒体混合功能氧化酶的能力分别与其非杂环类似物苯并(a)芘和苯并(a)蒽进行了比较。所有四种烃均显著增加了乙氧基试卤灵和乙氧基香豆素的O-脱乙基作用，非杂环类似物的作用更强。在戊氧基试卤灵的O-脱烷基作用中观察到较适度的增加。所有四种烃均诱导出可被细胞色素P-450IAI抗体识别的蛋白质，但使用细胞色素P-450IIB1抗体时未见增加。在艾姆斯试验中，所有预处理均增强了苯并(a)芘和Glu-P-1向诱变中间体的代谢活化作用。结论是，氮杂芳族多环烃与其非杂环类似物一样，选择性地诱导细胞色素P-450I家族的蛋白质。
