Karaman Ozan, Kelebek Seyfi, Demirci Emine Afra, İbiş Fatma, Ulu Murat, Ercan Utku Kürşat
1Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, Faculty of Engineering and Architecture, Rm 148, İzmir Katip Çelebi University, 35620 İzmir, Turkey.
2Department of Oral and Maxillofacial Surgery, İzmir Katip Çelebi University, 35620 İzmir, Turkey.
Tissue Eng Regen Med. 2017 Nov 10;15(1):13-24. doi: 10.1007/s13770-017-0087-5. eCollection 2018 Feb.
The aim of this study was to investigate the synergistic effect of cold atmospheric plasma (CAP) treatment and RGD peptide coating for enhancing cellular attachment and proliferation over titanium (Ti) surfaces. The surface structure of CAP-treated and RGD peptide-coated Ti discs were characterized by contact angle goniometer and atomic force microscopy. The effect of such surface modification on human bone marrow derived mesenchymal stem cells (hMSCs) adhesion and proliferation was assessed by cell proliferation and DNA content assays. Besides, hMSCs' adhesion and morphology on surface modified Ti discs were observed via fluorescent and scanning electron microscopy. RGD peptide coating following CAP treatment significantly enhanced cellular adhesion and proliferation among untreated, CAP-treated and RGD peptide-coated Ti discs. The treatment of Ti surfaces with CAP may contribute to improved RGD peptide coating, which enables increased cellular integrations with the Ti surfaces.
本研究的目的是探讨冷大气等离子体（CAP）处理与RGD肽涂层对增强钛（Ti）表面细胞附着和增殖的协同作用。通过接触角测量仪和原子力显微镜对CAP处理和RGD肽涂层的Ti盘的表面结构进行表征。通过细胞增殖和DNA含量测定评估这种表面修饰对人骨髓间充质干细胞（hMSCs）黏附及增殖的影响。此外，通过荧光显微镜和扫描电子显微镜观察hMSCs在表面修饰的Ti盘上的黏附及形态。CAP处理后进行RGD肽涂层，在未处理、CAP处理和RGD肽涂层的Ti盘中显著增强了细胞黏附及增殖。用CAP处理Ti表面可能有助于改善RGD肽涂层，从而增加细胞与Ti表面的整合。
