Onak Günnur, Karaman Ozan
Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, İzmir Katip Çelebi University, İzmir, Turkey.
Bonegraft Biomaterials Co., Ege University Technopolis, Bornova, İzmir, Turkey.
Regen Biomater. 2019 Aug;6(4):231-240. doi: 10.1093/rb/rbz014. Epub 2019 Apr 22.
Surface modification by non-thermal atmospheric plasma (NTAP) treatment can produce significantly higher carboxylic groups on the nanofibers (NF) surface, which potentially can increase biomineralization of NF via promoting glutamic acid (GLU) templated peptide conjugation. Herein, electrospun poly(lactide-co-glycolide) (PLGA) scaffolds were treated with NTAP and conjugated with GLU peptide followed by incubation in simulated body fluids for mineralization. The effect of NTAP treatment and GLU peptide conjugation on mineralization, surface wettability and roughness were investigated. The results showed that NTAP treatment significantly increased GLU peptide conjugation which consequently enhanced mineralization and mechanical properties of NTAP treated and peptide conjugated NF (GLU-pNF) compared to neat PLGA NF, NTAP treated NF (pNF) and GLU peptide conjugated NF (GLU-NF). The effect of surface modification on human bone marrow derived mesenchymal stem cells adhesion, proliferation and morphology was evaluated by cell proliferation assay and fluorescent microscopy. Results demonstrated that cellular adhesion and proliferation were significantly higher on GLU-pNF compared to NF, pNF and GLU-NF. In summary, NTAP treatment could be a promising modification technique to induce biomimetic peptide conjugation and biomineralization for bone tissue engineering applications.
通过非热大气等离子体（NTAP）处理进行表面改性可在纳米纤维（NF）表面产生显著更多的羧基，这有可能通过促进谷氨酸（GLU）模板化肽缀合来增加NF的生物矿化。在此，对静电纺聚（丙交酯 - 乙交酯）（PLGA）支架进行NTAP处理，并与GLU肽缀合，然后在模拟体液中孵育以进行矿化。研究了NTAP处理和GLU肽缀合对矿化、表面润湿性和粗糙度的影响。结果表明，与纯PLGA NF、NTAP处理的NF（pNF）和GLU肽缀合的NF（GLU - NF）相比，NTAP处理显著增加了GLU肽缀合，从而增强了NTAP处理和肽缀合的NF（GLU - pNF）的矿化和力学性能。通过细胞增殖测定和荧光显微镜评估了表面改性对人骨髓来源间充质干细胞粘附、增殖和形态的影响。结果表明，与NF、pNF和GLU - NF相比，GLU - pNF上的细胞粘附和增殖显著更高。总之，NTAP处理可能是一种有前景的改性技术，可用于骨组织工程应用中诱导仿生肽缀合和生物矿化。
