来自草莓假单胞菌IFO - 12049脂肪酶基因在大肠杆菌中的克隆、测序及表达
Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli.
作者信息
Aoyama S, Yoshida N, Inouye S
机构信息
Research Center, Chisso Petrochemical Corporation, Chiba, Japan.
出版信息
FEBS Lett. 1988 Dec 19;242(1):36-40. doi: 10.1016/0014-5793(88)80980-3.
PMID:3060375
Abstract
The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.
摘要
利用表达文库分离出了来自嗜冷假单胞菌IFO-12049的脂肪酶基因，并根据核苷酸序列推导确定了脂肪酶的一级结构。它由277个氨基酸残基组成，是一种分子量为29,966的蛋白质，这一数值与在大肠杆菌中表达的脂肪酶的值相近。
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