山矢车菊精油的体外细胞毒性和抗氧化特性
Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro.
作者信息
Çelikezen Fatih Çağlar, Hayta Şükrü, Özdemir Özlem, Türkez Hasan
机构信息
Department of Chemistry, Faculty of Science and Letters, Bitlis Eren University, Bitlis, Turkey.
Department of Environmental Engineering, Bitlis Eren University, Bitlis, Turkey.
出版信息
Cytotechnology. 2019 Feb;71(1):345-350. doi: 10.1007/s10616-018-0290-9. Epub 2019 Jan 2.
Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.
菊科矢车菊属植物在传统医学中被广泛使用。尽管矢车菊属植物有广泛的药用价值，但关于比亨矢车菊的毒性了解有限。因此，在本研究中，旨在确定比亨矢车菊精油对人血细胞培养物的细胞毒性和氧化作用。进行了3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和乳酸脱氢酶（LDH）释放试验以确定细胞毒性作用。此外，检测了总抗氧化能力（TAC）和总氧化状态（TOS）以确定氧化潜力。结果表明，所有测试浓度的比亨矢车菊精油均具有细胞毒性，并在两种试验中导致细胞活力下降。此外，比亨矢车菊导致TOS水平显著升高和TAC水平降低。总之，本研究首次表明比亨矢车菊精油对培养的人全血细胞具有细胞毒性和氧化作用。
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