收缩性平滑肌细胞中肌球蛋白磷酸化的空间模式：收缩区的证据。
Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones.
作者信息
Bennett J P, Cross R A, Kendrick-Jones J, Weeds A G
机构信息
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
出版信息
J Cell Biol. 1988 Dec;107(6 Pt 2):2623-9. doi: 10.1083/jcb.107.6.2623.
Abstract
We have purified a polyclonal antibody by affinity chromatography which binds specifically to the phosphorylated form of the regulatory light chain (Mr = 20,000) of smooth muscle myosin. This antibody does not stain relaxed, permeabilized smooth muscle cells isolated from guinea pig taenia coli. However, when these cells were stimulated to contract with CaCl2 (100 microM) and ATP (1 mM), the immunofluorescence staining was localized in a series of transverse bands. This distribution of activated myosin appears to reflect an underlying structural organization of the smooth muscle cell cytoskeleton into mechanically coupled contractile zones.
摘要
我们通过亲和层析纯化了一种多克隆抗体，该抗体能特异性结合平滑肌肌球蛋白调节轻链（分子量 = 20,000）的磷酸化形式。这种抗体不会对从豚鼠结肠带分离出的松弛、透化的平滑肌细胞进行染色。然而，当用氯化钙（100微摩尔）和ATP（1毫摩尔）刺激这些细胞收缩时，免疫荧光染色定位于一系列横向带中。活化肌球蛋白的这种分布似乎反映了平滑肌细胞细胞骨架在机械耦合收缩区的潜在结构组织。
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Proc Natl Acad Sci U S A. 1981 Aug;78(8):4738-42. doi: 10.1073/pnas.78.8.4738.
2
The binding of smooth muscle myosin light chain kinase to actin.
Biochem Biophys Res Commun. 1982 Aug 31;107(4):1524-31. doi: 10.1016/s0006-291x(82)80172-1.
3
Phosphorylation of nonmuscle myosin and stabilization of thick filament structure.
Methods Enzymol. 1982;85 Pt B:364-70. doi: 10.1016/0076-6879(82)85035-0.
4
Myosin filaments have non-phosphorylated light chains in relaxed smooth muscle.在舒张状态的平滑肌中，肌球蛋白丝含有非磷酸化轻链。
Nature. 1981 Dec 10;294(5841):567-9. doi: 10.1038/294567a0.
5
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J Biol Chem. 1981 Jul 25;256(14):7501-9.
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7
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