微流控芯片系统中的荧光寿命激活液滴分选。
Fluorescence lifetime-activated droplet sorting in microfluidic chip systems.
机构信息
Institute of Analytical Chemistry, Leipzig University, Linnéstraße 3, 04103 Leipzig, Germany.
出版信息
Lab Chip. 2019 Jan 29;19(3):403-409. doi: 10.1039/c8lc01278d.
We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on the basis of their corresponding fluorescence lifetime. Towards this end, a technical set-up was developed enabling on-the-fly fluorescence lifetime determination of passing droplets. The herein developed LabVIEW program enabled fast triggering of a downstream dielectrophoretic force sorting functionality depending on average fluorescence lifetimes of individual droplets. The approach worked reliably at individual substrate concentrations from 1 nM to 1 mM. This not only allowed reliable sorting of droplets containing species with different fluorescence lifetimes but also enabled differentiation of mixtures in individual droplets.
我们提出了一种高效的微流控荧光寿命激活液滴分选（FLADS）方法，作为一种在微流控芯片设备中进行液滴操作的新技术。在一项概念验证研究中，我们成功地应用该方法根据两种不同荧光化合物的相应荧光寿命对液滴进行分选。为此，我们开发了一种技术装置，能够对通过的液滴进行实时荧光寿命测定。本文开发的 LabVIEW 程序能够根据单个液滴的平均荧光寿命快速触发下游的介电泳力分选功能。该方法在 1 nM 至 1 mM 的单个基底浓度下可靠运行。这不仅允许对含有不同荧光寿命的物质的液滴进行可靠的分选，还允许在单个液滴中对混合物进行区分。
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