1Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
2National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Mar;69(3):727-731. doi: 10.1099/ijsem.0.003214. Epub 2019 Jan 3.
A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain HR-AS, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were motile rods with a polar flagellum. Growth was observed at 5-35 °C (optimum of 25 °C) and pH 6-8 (optimum of pH 7) and in the presence of 0-2 % (w/v) NaCl (optimum of 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AS formed a tight phylogenic lineage with Zavarzinia compransoris LMG 5821. Strain HR-AS was most closely related to Z. compransoris LMG 5821 with a 98.7 % 16S rRNA gene sequence similarity and had very low similarities (below 91.0 %) to other type strains with validly published names. Average nucleotide identity and in silico DNA-DNA hybridization values between strain HR-AS and Z. compransoris DSM 1231 were 80.4 and 23.1 %, respectively. Strain HR-AS contained ubiquinone-10 as the major quinone and homospermidine and putrescine as the major polyamines. The major fatty acids were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c), C16 : 0 and C18 : 1 2-OH. Strain HR-AS contained diphosphatidylglycerol, an unidentified aminolipid and two unidentified phospholipids as major polar lipids. The DNA G+C content of strain HR-AS was 67.2 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-AS represents a novel species of the genus Zavarzinia, for which the name Zavarziniaaquatilis sp. nov. is proposed. The type strain is HR-AS (=KACC 19412=JCM 32263).
一株革兰氏阴性、严格需氧、接触酶阳性和氧化酶阳性的细菌，被命名为 HR-AS 菌株，从韩国汉江的一个水样中分离得到。细胞是具有极生鞭毛的运动杆状菌。该菌在 5-35°C（最适 25°C）和 pH 6-8（最适 pH 7）以及 0-2%（w/v）NaCl（最适 0%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HR-AS 与 Zavarzinia compransoris LMG 5821 形成了一个紧密的系统发育分支。菌株 HR-AS 与 Z. compransoris LMG 5821 的 16S rRNA 基因序列相似性最高，为 98.7%，与其他具有有效发表名称的模式菌株的相似性非常低（低于 91.0%）。菌株 HR-AS 与 Z. compransoris DSM 1231 之间的平均核苷酸同一性和 DNA-DNA 杂交值分别为 80.4%和 23.1%。菌株 HR-AS 含有泛醌-10 作为主要的醌类化合物，腐胺和亚精胺作为主要的多胺类化合物。主要脂肪酸为 sum 功能 8（C18：1 ω6c 和/或 C18：1 ω7c）、C16：0 和 C18：1 2-OH。菌株 HR-AS 含有二磷脂酰甘油、一种未鉴定的氨基脂类和两种未鉴定的磷脂类作为主要极性脂类。菌株 HR-AS 的 DNA G+C 含量为 67.2 mol%。基于基因型、化学分类和表型分析，菌株 HR-AS 代表 Zavarzinia 属的一个新种，建议将其命名为 Zavarzinia aquatilis sp. nov.。模式菌株为 HR-AS（=KACC 19412=JCM 32263）。
