Khan Shehzad Abid, Jeong Sang Eun, Jung Hye Su, Quan Zhe-Xue, Jeon Che Ok
1Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
2School of Life Sciences, Fudan University, Shanghai 200438, PR China.
Int J Syst Evol Microbiol. 2019 May;69(5):1384-1389. doi: 10.1099/ijsem.0.003322. Epub 2019 Feb 28.
A Gram-stain-negative, facultatively aerobic bacterial strain, designated DB1506, of the family Acetobacteraceae, was isolated from an air-conditioning system in the Republic of Korea. Colonies were pink- to rosy-coloured and cells were non-motile cocci with catalase- and oxidase-positive activities. Growth of strain DB1506 was observed at 20-37 °C (optimum, 30 °C), pH 5.5-8.5 (pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl (0 %). Strain DB1506 contained summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C18 : 1 2-OH as major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipids, unidentified aminolipids and unidentified polar lipids were detected as major polar lipids. The G+C content of the genomic DNA calculated from the whole genome sequence was 72.5 mol%. Strain DB1506 was most closely related to Paracraurococcus ruber NS89, Dankookia rubra WS-10 and Siccirubricoccus deserti SYSU D8009 with 16S rRNA gene sequence similarities of 96.01, 95.88 and 95.44 %, respectively, but strain DB1506 formed a clearly distinct phylogenic lineage from them within the family Acetobacteraceae. On the basis of phenotypic, chemotaxonomic and molecular properties, strain DB1506 represents a novel species of a new genus within the family Acetobacteraceae, for which the name Roseicella frigidaeris gen. nov., sp. nov. is proposed. The type strain is DB1506 (=KACC 19791=JCM 32945).
从韩国的一个空调系统中分离出一株革兰氏阴性、兼性需氧细菌菌株，命名为DB1506，属于醋杆菌科。菌落呈粉红色至玫瑰色，细胞为无运动性的球菌，过氧化氢酶和氧化酶呈阳性。在20-37°C（最适温度为30°C）、pH 5.5-8.5（pH 7.0）以及0-0.5%（w/v）NaCl（0%）存在的条件下观察到菌株DB1506的生长。菌株DB1506以特征性总和8（包含C18:1ω7c和/或C18:1ω6c）和C18:1 2-OH作为主要脂肪酸，以泛醌-10作为唯一的类异戊二烯醌。检测到磷脂酰甘油、磷脂酰乙醇胺、未鉴定的磷脂、未鉴定的氨基脂质和未鉴定的极性脂质为主要极性脂质。根据全基因组序列计算的基因组DNA的G+C含量为72.5mol%。菌株DB1506与微红副嗜冷球菌NS89、深红丹国菌WS-10和沙漠嗜红球菌SYSU D8009关系最为密切，16S rRNA基因序列相似性分别为96.01%、95.88%和95.44%，但菌株DB1506在醋杆菌科内与它们形成了明显不同的系统发育分支。基于表型、化学分类和分子特性，菌株DB1506代表醋杆菌科内一个新属的一个新物种，为此提出新属名玫瑰冷杆菌属（Roseicella），新种名冷栖玫瑰冷杆菌（Roseicella frigidaeris）。模式菌株为DB1506（=KACC 19791=JCM 32945）。
