Kizhakeyil Atish, Ong Seow Theng, Fazil Mobashar Hussain Urf Turabe, Chalasani Madhavi Latha Somaraju, Prasannan Praseetha, Verma Navin Kumar
Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.
Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, NY, USA.
Methods Mol Biol. 2019;1930:11-17. doi: 10.1007/978-1-4939-9036-8_2.
Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.
外周血是体外培养T淋巴细胞最常见的来源。在此，我们介绍一种简单且标准化的方法，用于从新鲜外周血或血沉棕黄层血样中，通过密度梯度离心小规模或大规模分离活的T淋巴细胞和其他单核细胞。使用本文所述方案获得的T细胞可用于多种下游分析，包括细胞、分子和功能检测。
