Terness P, Schiffl R, Süsal C, Opelz G
Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Federal Republic of Germany.
Transplantation. 1988 Dec;46(6):812-9. doi: 10.1097/00007890-198812000-00005.
Transfusion with antibody-coated allogeneic blood cells suppresses the cytotoxic antidonor antibody response in a strongly incompatible rat combination (BN----LEW). Cell coating with homologous recipient antidonor antiserum, rat monoclonal antibodies against MHC class I donor antigens, or rabbit antirat lymphocyte serum all were effective. The suppression was not abrogated by repeated booster transfusions with untreated donor blood. Moreover, the suppression extended to antibody-uncoated antigens present on the same donor cell. Not only the antibody response but also the Graft-versus-host reaction against donor antigens was suppressed. The serum of pretreated animals contained suppressive activity. It suppressed the cytotoxic antibody response as well as the cellular immune response (GVH) when transferred into syngeneic recipients. A weaker suppression of antibody response was obtained by transferring spleen cells of pretreated animals into syngeneic recipients. The transfer data suggest that broadly reactive serum factor(s) were mainly responsible for the suppressive effect. Transfusion with LEW-anti-BN-coated donor cells before transplantation induced markedly prolonged kidney graft survival in the BN----LEW combination without additional immunosuppression (untreated controls: 8.4 +/- 0.4, pretreated recipients: 124 +/- 36 days, P less than 10(-4)).
输注抗体包被的同种异体血细胞可抑制强不相容大鼠组合（BN----LEW）中的细胞毒性抗供体抗体反应。用同源受体抗供体抗血清、针对MHC I类供体抗原的大鼠单克隆抗体或兔抗大鼠淋巴细胞血清包被细胞均有效。用未处理的供体血液反复进行加强输血并不能消除这种抑制作用。此外，这种抑制作用还扩展到同一供体细胞上存在的未被抗体包被的抗原。不仅抗体反应受到抑制，针对供体抗原的移植物抗宿主反应也受到抑制。预处理动物的血清具有抑制活性。当将其转移到同基因受体中时，它可抑制细胞毒性抗体反应以及细胞免疫反应（GVH）。将预处理动物的脾细胞转移到同基因受体中可获得较弱的抗体反应抑制作用。转移数据表明，具有广泛反应性的血清因子主要负责这种抑制作用。在移植前输注LEW抗BN包被的供体细胞可显著延长BN----LEW组合中肾脏移植物的存活时间，而无需额外的免疫抑制（未处理对照组：8.4 +/- 0.4天，预处理受体：124 +/- 36天，P小于10(-4)）。
