Hurme M, Palkama T, Sihvola M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):861-6. doi: 10.1016/s0006-291x(88)80954-9.
T cell derived cytokines, interleukin-2 and interferon-gamma, are known to upregulate the interleukin-1 synthesis of monocyte/macrophages. The effect of a third T cell derived cytokine, interleukin-4, was now investigated. Human peripheral blood monocytes were activated with bacterial lipopolysaccharide in the presence or absence of interleukin-4 and the production of biologically active interleukin-1 was quantitated both from the culture supernatants and from the monocyte lysates using the mouse thymocyte assay. Interleukin-4 had a clear dose-dependent suppressive effect on the interleukin-1 synthesis. IL-1 alpha and IL-1 beta mRNA levels, quantitated by Northern blot analysis, were equally high in the lipopolysaccharide plus interleukin-4 supplemented cultures and in the cultures with lipopolysaccharide alone, suggesting that the effect of interleukin-4 in the interleukin-1 biosynthesis is post-transcriptional.
已知T细胞衍生的细胞因子白细胞介素-2和干扰素-γ可上调单核细胞/巨噬细胞的白细胞介素-1合成。现在研究了第三种T细胞衍生细胞因子白细胞介素-4的作用。在存在或不存在白细胞介素-4的情况下，用人外周血单核细胞与细菌脂多糖激活，使用小鼠胸腺细胞测定法从培养上清液和单核细胞裂解物中定量生物活性白细胞介素-1的产生。白细胞介素-4对白细胞介素-1合成具有明显的剂量依赖性抑制作用。通过Northern印迹分析定量的IL-1α和IL-1βmRNA水平在脂多糖加白细胞介素-4补充的培养物中和仅含脂多糖的培养物中同样高，表明白细胞介素-4在白细胞介素-1生物合成中的作用是转录后水平的。
