Marre R, Schulz E
Institut für Medizinische Mikrobiologie, Medizinischen Universität zu Lübeck, Fed. Rep. of Germany.
Arzneimittelforschung. 1988 Jul;38(7):863-5.
The in vitro activity of mecillinam and amoxicillin/clavulanic acid against Escherichia coli strains producing beta-lactamases of the TEM-1, Oxa-1 and chromosomal type were studied using the broth and agar dilution technique. In addition the beta-lactamase activity was determined. The experiments indicated that the beta-lactamase types differed in their influence on the minimum inhibitory concentrations. Both, mecillinam and amoxicillin/clavulanic acid were considerably more active against TEM-1 strains than ampicillin alone, but, with increasing specific beta-lactamase activity, a decrease of susceptibility was found. Strains producing Oxa-1 and chromosomal beta-lactamases usually where highly susceptible to mecillinam but only moderately sensitive or resistant to the combination of amoxicillin/clavulanic acid. In vitro activity of TEM-1 E. coli to mecillinam was strongly reduced when tested in a broth dilution technique. These test systems, however, did not affect the susceptibility of Oxa-1 and chromosomal beta-lactamase producing E. coli strains to either antibiotic.
采用肉汤稀释法和琼脂稀释法，研究了美西林和阿莫西林/克拉维酸对产TEM-1、Oxa-1和染色体型β-内酰胺酶的大肠杆菌菌株的体外活性。此外，还测定了β-内酰胺酶活性。实验表明，β-内酰胺酶类型对最低抑菌浓度的影响不同。美西林和阿莫西林/克拉维酸对TEM-1菌株的活性均比单独使用氨苄西林高得多，但随着特异性β-内酰胺酶活性的增加，敏感性降低。产Oxa-1和染色体β-内酰胺酶的菌株通常对美西林高度敏感，但对阿莫西林/克拉维酸组合仅中度敏感或耐药。当采用肉汤稀释法检测时，TEM-1大肠杆菌对美西林的体外活性显著降低。然而，这些检测系统并不影响产Oxa-1和染色体β-内酰胺酶的大肠杆菌菌株对任何一种抗生素的敏感性。
