Department of Applied Chemistry, College of Science , China Agricultural University , Beijing 100193 , China.
Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine , University of Florida , Gainesville , Florida 32611 , United States.
Environ Sci Technol. 2019 Feb 5;53(3):1672-1679. doi: 10.1021/acs.est.8b05829. Epub 2019 Jan 16.
The strobilurin fungicide azoxystrobin (AZO) can induce adverse effects in aquatic organisms, but data are lacking on endpoints associated with sexual development and reproduction following chronic exposure to AZO. In this study, zebrafish embryos (F0) at 2-4 h postfertilization (hpf) were exposed to 0.2, 2.0, and 20.0 μg/L AZO until 120 d postfertilization (dpf). Decreased male ratio and increased intersex ratio were observed by 20.0 μg/L AZO at 42 and 60 dpf, but this effect disappeared at 120 dpf. AZO at 20.0 μg/L inhibited growth, retarded gonadal development, and disrupted sex hormone and vitellogenin in females at 60 and 120 dpf and in males at 42, 60, and 120 dpf. These effects were associated with altered expression of cyp19a, cyp19b, hsd3b, hsd17b, vtg1, and vtg2. Exposure to 2.0 μg/L AZO altered mRNA levels of these transcripts in females at 120 dpf and in males at 60 and 120 dpf. Reproduction ability was reduced by 20.0 μg/L AZO at 120 dpf. Developmental defects were observed after F1 embryos from exposed parents of 20.0 μg/L were reared in AZO-free water at 96 hpf. Overall, these data provide new understanding of fish sexual development and reproduction following chronic exposures to AZO.
唑菌胺酯（AZO）是一种苯并咪唑类杀菌剂，能诱导水生生物产生不良反应，但目前缺乏唑菌胺酯慢性暴露后与生殖发育相关的终点数据。本研究中，将受精后 2-4 小时（hpf）的斑马鱼胚胎暴露于 0.2、2.0 和 20.0 μg/L AZO 中直至受精后 120 天（dpf）。在 42 和 60 dpf 时，20.0 μg/L AZO 导致雄性比例降低，雌雄间性比例增加，但在 120 dpf 时这种影响消失。20.0 μg/L AZO 抑制生长，延迟性腺发育，并在 60 和 120 dpf 的雌性和 42、60 和 120 dpf 的雄性中扰乱性激素和卵黄蛋白原。这些影响与 cyp19a、cyp19b、hsd3b、hsd17b、vtg1 和 vtg2 的表达改变有关。2.0 μg/L AZO 暴露在 120 dpf 的雌性和 60 和 120 dpf 的雄性中改变了这些转录物的 mRNA 水平。20.0 μg/L AZO 在 120 dpf 时降低了繁殖能力。暴露于 20.0 μg/L AZO 的亲鱼产生的 F1 胚胎在无 AZO 水中培养至 96 hpf 时观察到发育缺陷。总之，这些数据为唑菌胺酯慢性暴露后鱼类生殖发育提供了新的认识。
