Department of Chemistry , ICMUB , 9 avenue Alain Savary , 21000 Dijon , France.
Laboratoire d'Immunologie et Immunothérapie des Cancers, EPHE , PSL Research University , 75000 Paris , France.
Bioconjug Chem. 2019 Apr 17;30(4):1061-1066. doi: 10.1021/acs.bioconjchem.8b00795. Epub 2019 Jan 22.
A new family of water-soluble and bioconjugatable aza-BODIPY fluorophores was designed and synthesized using a boron- functionalization strategy. These dissymmetric bis-ammonium aza-BODIPY dyes present optimal properties for a fluorescent probe; i.e., they are highly water-soluble, very stable in physiological medium; they do not aggregate in PBS, possess high quantum yield; and finally, they can be easily bioconjugated to antibodies. Preliminary in vitro and in vivo studies were performed for one of these fluorophores to image PD-L1 (Programmed Death-Ligand 1), highlighting the high potential of these new probes for future in vivo optical imaging studies.
设计并合成了一类新型的水溶性和可生物偶联的氮杂 BODIPY 荧光染料，该染料采用硼功能化策略。这些不对称双铵氮杂 BODIPY 染料是理想的荧光探针，具有高水溶性、在生理介质中非常稳定、在 PBS 中不聚集、高量子产率和易于与抗体进行生物偶联等特点。对其中一种荧光染料进行了初步的体外和体内研究，以对 PD-L1（程序性死亡配体 1）进行成像，突出了这些新型探针在未来体内光学成像研究中的巨大潜力。
