Kratochwil K
Institut für Molekularbiologie der Osterreichischen Akademie der Wissenschaften, Salzburg, Austria.
Cell Differ Dev. 1988 Nov;25 Suppl:119-26. doi: 10.1016/0922-3371(88)90108-6.
The collagen I-deficient mouse mutant (Mov13 - an embryonic recessive lethal) was used to investigate the function of this major constituent of the extracellular matrix (ECM) in organ development. All epithelial-mesenchymal organs tested as explants (lung, kidney, pancreas, salivary glands, skin) developed normally and, in particular, showed typical branching morphogenesis in the absence of collagen I. It is concluded that the ECM of these organs can organize for proper developmental function in the absence of the major interstitial collagen, but a possible morphogenetic function of other fibrillar collagens (types III and V) cannot be excluded. The only insufficiencies in the mutant were seen in the cornea where deposition and organization of the collagenous stroma was highly inadequate; but even there, development and migration of cells proceeded normally. In summary, the results indicate that 'cellular' development in epithelial-mesenchymal organs (including growth, morphogenesis, and differentiation) does not depend on collagen I.
利用缺乏I型胶原蛋白的小鼠突变体（Mov13——一种胚胎隐性致死突变体）来研究这种细胞外基质（ECM）的主要成分在器官发育中的功能。作为外植体进行测试的所有上皮-间充质器官（肺、肾、胰腺、唾液腺、皮肤）均正常发育，特别是在缺乏I型胶原蛋白的情况下表现出典型的分支形态发生。得出的结论是，在缺乏主要的间质胶原蛋白的情况下，这些器官的细胞外基质仍能组织起来发挥正常的发育功能，但不能排除其他纤维状胶原蛋白（III型和V型）可能具有的形态发生功能。在突变体中，唯一出现不足的部位是角膜，其中胶原基质的沉积和组织非常不足；但即便如此，细胞的发育和迁移仍正常进行。总之，结果表明上皮-间充质器官中的“细胞性”发育（包括生长、形态发生和分化）并不依赖于I型胶原蛋白。
