单细胞测序的应用。
Single-Cell Applications of Next-Generation Sequencing.
机构信息
Department of Molecular and Cellular Biology, Stony Brook University, Stony Brook, New York 11794.
Watson School of Biological Science, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724.
出版信息
Cold Spring Harb Perspect Med. 2019 Oct 1;9(10):a026898. doi: 10.1101/cshperspect.a026898.
The single cell is considered the basic unit of biology, and the pursuit of understanding how heterogeneous populations of cells can functionally coexist in tissues, organisms, microbial ecosystems, and even cancer, makes them the subject of intense study. Next-generation sequencing (NGS) of RNA and DNA has opened a new frontier of (single)-cell biology. Hundreds to millions of cells now can be assayed in parallel, providing the molecular profile of each cell in its milieu inexpensively and in a manner that can be analyzed mathematically. The goal of this article is to provide a high-level overview of single-cell sequencing for the nonexpert and show how its applications are influencing both basic and applied clinical studies in embryology, developmental genetics, and cancer.
单细胞被认为是生物学的基本单位，而探索如何使组织、生物体、微生物生态系统，甚至癌症中的异质细胞群体能够协同发挥功能，这使得单细胞成为了研究的热点。RNA 和 DNA 的新一代测序（NGS）为（单细胞）生物学开辟了一个新的领域。现在可以同时分析数百到数百万个细胞，以经济且可进行数学分析的方式提供每个细胞在其微环境中的分子图谱。本文的目的是为非专业人士提供单细胞测序的高级概述，并展示其应用如何影响胚胎学、发育遗传学和癌症的基础和应用临床研究。
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