综合/混合方法结构生物学：大分子晶体学的作用。

Integrative/Hybrid Methods Structural Biology: Role of Macromolecular Crystallography.

机构信息

Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

出版信息

Adv Exp Med Biol. 2018;1105:11-18. doi: 10.1007/978-981-13-2200-6_2.

Abstract

Macromolecular crystallography has been central to the emergence and development of structural biology as a scientific discipline. Approximately 90% of the more than 138,000 three-dimensional structures currently available in the Protein Data Bank (PDB) archive, the single, global open access data resource for macromolecular structure data, were determined using X-ray crystallography. MX, the enormous variety of PDB structures of proteins, DNA, and RNA, and computational models derived therefrom will be central to the growth of integrative or hybrid (I/H) methods structural studies of macromolecular assemblies and other complex biological systems.

摘要

大分子晶体学一直是结构生物学作为一门科学学科出现和发展的核心。目前，在蛋白质数据银行（PDB）档案中，约有 138000 多个三维结构，其中 90%以上是使用 X 射线晶体学确定的。MX 是蛋白质、DNA 和 RNA 的 PDB 结构的巨大多样性，以及由此衍生的计算模型，将成为大分子组装和其他复杂生物系统的综合或混合（I/H）方法结构研究的核心。