PA 标签：整合结构生物学时代的多功能肽标签系统。
The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology.
机构信息
Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
出版信息
Adv Exp Med Biol. 2018;1105:59-76. doi: 10.1007/978-981-13-2200-6_6.
We have recently developed a novel protein tagging system based on the high affinity interaction between an antibody NZ-1 and its antigen PA peptide, a dodecapeptide that forms a β-turn in the binding pocket of NZ-1. This unique conformation allows for the PA peptide to be inserted into turn-forming loops within a folded protein domain and the system has been variously used in general applications including protein purification, Western blotting and flow cytometry, or in more specialized applications such as reporting protein conformational change, and identifying subunits of macromolecular complexes with electron microscopy. Thus the small and "portable" nature of the PA tag system offers a versatile and powerful tool that can be implemented in various aspects of integrative structural biology.
我们最近开发了一种新颖的蛋白质标记系统，该系统基于抗体 NZ-1 与其抗原 PA 肽之间的高亲和力相互作用，PA 肽是一个形成 NZ-1 结合口袋中β-转角的十二肽。这种独特的构象允许 PA 肽插入折叠蛋白质结构域中的转角形成环，该系统已广泛用于一般应用，包括蛋白质纯化、Western 印迹和流式细胞术，或更专门的应用，如报告蛋白质构象变化，以及使用电子显微镜鉴定大分子复合物的亚基。因此，PA 标签系统的小巧和“可移植”性质提供了一种多功能且强大的工具，可以在整合结构生物学的各个方面实施。
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