Rajkowska Grazyna, Miguel-Hidalgo Jose Javier
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, MS, USA.
Methods Mol Biol. 2019;1938:247-254. doi: 10.1007/978-1-4939-9068-9_17.
Double immunohistochemistry and confocal microscopy permits to detect the coverage of blood vessels by astrocytic endfeet in human postmortem brain tissue. Here we describe double immunofluorescent staining for detecting a colocalization of a marker of astrocytic processes (labeled with an antibody for aquaporin-4 (AQP4) and a marker for blood vessels (labeled with an antibody to collagen IV). Then, we present a microscopic analysis of the coverage of blood vessels by astrocytic processes using Nikon C1 confocal microscope, Photoshop, and ImageJ software.
双重免疫组织化学和共聚焦显微镜技术能够检测人死后脑组织中星形胶质细胞终足对血管的覆盖情况。在此，我们描述用于检测星形胶质细胞突起标志物（用抗水通道蛋白4（AQP4）抗体标记）和血管标志物（用抗IV型胶原抗体标记）共定位的双重免疫荧光染色。然后，我们使用尼康C1共聚焦显微镜、Photoshop和ImageJ软件对星形胶质细胞突起对血管的覆盖情况进行显微镜分析。
