Mudd E A, Prentki P, Belin D, Krisch H M
Department of Molecular Biology, University of Geneva, Switzerland.
EMBO J. 1988 Nov;7(11):3601-7. doi: 10.1002/j.1460-2075.1988.tb03238.x.
Gene 32 from bacteriophage T4 is transcribed as precursor transcripts which are processed to a stable product. This processing of the gene 32 mRNA was observed in RNase III or P-deficient strains of Escherichia coli. However, after infection of an RNase E-deficient strain, the amount of processed transcript was significantly reduced while the levels of the precursor transcripts remained high. RNase E therefore appears to have an essential role in the processing of the gene 32 mRNA. We have mapped the exact 5' end of the processed transcript by primer extension. The cleavage occurs near a stem-loop structure at a site which shows some similarity to other known RNase E cleavage sites. The effects of the processing on the differential stability of the upstream and downstream sequences, and on gene expression, are discussed.
噬菌体T4的基因32转录为前体转录本，这些转录本会被加工成稳定的产物。在大肠杆菌的RNase III或P缺陷菌株中观察到了基因32 mRNA的这种加工过程。然而，在感染RNase E缺陷菌株后，加工后的转录本数量显著减少，而前体转录本的水平仍然很高。因此，RNase E似乎在基因32 mRNA的加工过程中起着至关重要的作用。我们通过引物延伸确定了加工后转录本的确切5'端。切割发生在一个茎环结构附近的位点，该位点与其他已知的RNase E切割位点有一些相似之处。本文讨论了加工对上游和下游序列差异稳定性以及基因表达的影响。
