胰岛素与实验性诱导的慢性高催乳素血症雌性大鼠脂肪细胞的结合及作用

Insulin binding and action on adipocytes from female rats with experimentally induced chronic hyperprolactinemia.

作者信息

Cabrera R, Mayor P, Fernández-Ruiz J, Calle C

机构信息

Department of Biochemistry, Faculty of Medicine, Complutense University, Madrid, Spain.

出版信息

Mol Cell Endocrinol. 1988 Aug;58(2-3):167-73. doi: 10.1016/0303-7207(88)90151-7.

DOI:10.1016/0303-7207(88)90151-7

PMID:3061857

Abstract

We studied insulin binding and action in adipocytes from female rats with chronic hyperprolactinemia induced by grafting an anterior pituitary gland under the right kidney capsule. Normal basal insulin plasma levels were detected. An increase in insulin binding due to an increased number of receptors was observed (grafted: 193,000 +/- 13,000 (6) receptors/cell vs. controls: 136,000 +/- 17,000 (6) receptors/cell, P less than 0.05). No changes in receptor affinity were detected (ED50 grafted: 2.3 X 10(-9) M and ED50 controls: 1.6 X 10(-9) M). The antilipolytic activity of insulin was significantly decreased in adipocytes from rats with hyperprolactinemia, indicating an insulin-resistant state in these animals. These findings suggest that the chronic hyperprolactinemic state can modify receptor and post-receptor insulin events in rat parametrial adipose tissue.

摘要

我们研究了通过将垂体移植到右肾包膜下诱导慢性高催乳素血症的雌性大鼠脂肪细胞中的胰岛素结合和作用。检测到正常的基础胰岛素血浆水平。观察到由于受体数量增加导致胰岛素结合增加（移植组：193,000±13,000（6）个受体/细胞，对照组：136,000±17,000（6）个受体/细胞，P<0.05）。未检测到受体亲和力的变化（移植组的半数有效剂量：2.3×10^(-9) M，对照组的半数有效剂量：1.6×10^(-9) M）。高催乳素血症大鼠脂肪细胞中胰岛素的抗脂解活性显著降低，表明这些动物存在胰岛素抵抗状态。这些发现表明，慢性高催乳素血症状态可改变大鼠子宫旁脂肪组织中受体及受体后胰岛素事件。