巴西首次观察到 Merkel 细胞多瘤病毒与 Merkel 细胞癌的关联。
The first observation of the association of Merkel cell polyomavirus and Merkel cell carcinoma in Brazil.
机构信息
Department of Dermatology, Clinics Hospital of the Medical School, University of São Paulo, São Paulo, Brazil.
Virology Medical Research Laboratory, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.
出版信息
Int J Dermatol. 2019 Jun;58(6):703-706. doi: 10.1111/ijd.14325. Epub 2019 Jan 8.
BACKGROUND
Merkel cell carcinoma (MCC) is a rare but aggressive primary cutaneous carcinoma with high mortality rates. The present study intends to delineate the epidemiological profile of patients with MCC seen at the Clinics Hospital of the Medical School at the University of São Paulo, Brazil, and its association with Merkel cell polyomavirus (MCPyV).
METHODS
This is a retrospective study. A search was performed in the hospital's medical index for all cases of MCC from January 1994 to December 2012. Among patients with MCC, the available tumoral skin specimens were analyzed with two different techniques of polymerase chain reaction (PCR) (conventional and real-time) for detection of MCPyV DNA. Additionally, paraffin-embedded samples of patients with non-MCC skin cancers were also analyzed. Analyses suitable for categorical data (i.e., x² of Fisher) were used to compare the proportion of patients in each group.
RESULTS
Nineteen patients with MCC and 20 patients with non-MCC skin cancers entered the study. All MCC samples available (13) tested positive for the presence of MCPyV DNA; however, in the non-MCC skin cancer samples, the MCPyV DNA was detected in 4 of 20 samples (20%). MCPyV DNA detection rate was higher in patients with MCC than in the other group, and its analysis was statistically significant (P < 0.01).
CONCLUSIONS
This study demonstrates the association of MCPyV in Brazilian patients with MCC. However, further studies are necessary to determine the exact involvement of MCPyV in MCC pathogenesis and to define the significance of viral DNA detection in non-MCC skin cancers.
背景
默克尔细胞癌（MCC）是一种罕见但具有侵袭性的皮肤原发性癌，死亡率较高。本研究旨在描述巴西圣保罗医学院附属医院皮肤科收治的 MCC 患者的流行病学特征及其与 Merkel 细胞多瘤病毒（MCPyV）的关系。
方法
这是一项回顾性研究。对 1994 年 1 月至 2012 年 12 月期间所有 MCC 患者的医院医疗索引进行了检索。在 MCC 患者中，对可用的肿瘤皮肤标本进行了两种不同聚合酶链反应（PCR）技术（常规和实时）检测 MCPyV DNA。此外，还对非 MCC 皮肤癌患者的石蜡包埋样本进行了分析。适用于分类数据（即 Fisher's x²）的分析用于比较每组患者的比例。
结果
19 例 MCC 患者和 20 例非 MCC 皮肤癌患者进入研究。所有可获得的 MCC 样本（13 例）均检测到存在 MCPyV DNA；然而，在非 MCC 皮肤癌样本中，20 例样本中有 4 例（20%）检测到 MCPyV DNA。MCC 患者中 MCPyV DNA 的检出率高于其他组，且分析具有统计学意义（P<0.01）。
结论
本研究表明 MCPyV 与巴西 MCC 患者有关。然而，需要进一步的研究来确定 MCPyV 在 MCC 发病机制中的确切作用，并确定非 MCC 皮肤癌中病毒 DNA 检测的意义。
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