Mahany T M, Parsons R H
Am J Physiol. 1978 May;234(5):R172-7. doi: 10.1152/ajpregu.1978.234.5.R172.
Tritiated water (3H2O) exchange was shown to be more sensitive to circulatory changes than to arginine vasopressin (ADH)-induced permeability changes. Reducing the circulation (sciatic artery ligation) and increasing the circulation (severing the sciatic nerve) caused the 3H2O exchange in frog legs to decrease from 6.0 +/- 1.3 (5) to 3.5 +/- 0.5 and increase from 6.0 +/- 0.7 (10) to 8.6 +/- 0.6 ml.h-1, respectively. In contrast injection of ADH caused a negligible increase in 3H2O exchange from whole frogs, while at the same time causing a significant increase in osmotic water exchange from 0.22 +/- 0.08 (6) to 0.63 +/- 0.13 (6) g.100 cm-2.h-1.200 mosM-1. Circulatory changes in whole frogs were produced by inducing a diving bradycardia that was less pronounced in a bath aerated with oxygen than with air. The bradycardia caused a reduction in skin circulation, as measured by 3H2O exchange, from 0.210 +/- 0.007 (16) to 0.17 +/- 0.006 (16) ml.h-1.cm-2 in air mixed media and from 0.149 +/- 0.009 (16) to 0.135 +/- 0.011 (16) in oxygen mixed media. Diving bradycardia was also found to affect osmotic water uptake which decreased from 0.61 +/- 0.05 (16) to 0.39 +/- 0.03 (16) g.100 cm-2.h-1.200 mosM-1 in air mixed media and from 0.61 +/- 0.05 (16) to 0.51 +/- 0.04 (16) in oxygen mixed media. The results indicate that circulation affects osmotic water exchange.
氚水（3H2O）交换对循环变化的敏感性高于对精氨酸加压素（ADH）诱导的通透性变化的敏感性。减少循环（结扎坐骨动脉）和增加循环（切断坐骨神经）分别导致蛙腿的3H2O交换量从6.0±1.3（5）降至3.5±0.5，以及从6.0±0.7（10）增至8.6±0.6 ml·h-1。相比之下，注射ADH导致全蛙的3H2O交换量增加可忽略不计，而同时导致渗透水交换量从0.22±0.08（6）显著增至0.63±0.13（6）g·100 cm-2·h-1·200 mosM-1。通过诱导潜水性心动过缓在全蛙中产生循环变化，在充氧浴中比在空气浴中这种心动过缓不太明显。如通过3H2O交换所测，心动过缓导致皮肤循环减少，在空气混合介质中从0.210±0.007（16）降至0.17±0.006（16）ml·h-1·cm-2，在氧气混合介质中从0.149±0.009（16）降至0.135±0.011（16）。还发现潜水性心动过缓会影响渗透水摄取，在空气混合介质中从0.61±0.05（16）降至0.39±0.03（16）g·100 cm-2·h-1·200 mosM-1，在氧气混合介质中从0.61±0.05（16）降至0.51±0.04（16）。结果表明循环会影响渗透水交换。
