[THE BIOFILM FORMATION ABILITY OF STRAINS SERRATIA SPP., SEPARATED FROM WOUNDS OF PATIENTS WITH CHRONIC OSTEOMYELITIS IN MONO-CULTURES AND IN COMPOSITION OF ASSOCIATION OF MICROORGANISMS HARVESTED IN VITRO].

The article presents analysis of characteristics of 7 clinical strains of Serratia marcescens separated from fistulas in pre-operational period and from nidus of inflammation during operations in 2013-2015. in 7 patients with chronic osteomyelitis of long tubular bones. The bacteria S.marcescens are separated in one patient in monoculture and in 6 patients in composition of associations: S.marcescens + S.warneri + P.aeruginosa (n=1); S.marcescens + S.aureus (n=4); S.marcescens + M.morganii (n=1). According phometric analysis, strains of S.marcescens were characterized by average ability for biofilm formation on the surfaces of polystyrene dishes and cover glass that is substantiated by values of optic density and is conformed to data of their adhesion activity. The biofilm formation ability of association of microorganisms (S.marcescens + P.aeruginosa, S.marcescens + M.morgani) already after 24 hours of experiment were correspondingly higher in 1.4 and 1.2 times of levels of biofilm formation in monocultures. The activity of biofilm formation of association (S.marcescens + S.aureus) on the first day of experiment was low. However, after 48 hours a significant growth of biofilm was observed that is substantiated by values of optical density. The results of study demonstrated ability of all clinical strains of S.marcescens separated from osteomyelitis nidus to adhesion on surface of erythrocytes and to biofilm formation on abiotic surfaces (polystyrene and glass) both in monoculture and associations with other microorganisms. In consideration that microbial films play leading role in chronization of infectious diseases it is necessary to be aware of seriousness of etiologic role of S.marcescens in development of osteomyelitis as a pathogen.