人牙龈组织来源间充质干细胞通过 CD39 途径减轻小鼠急性移植物抗宿主病的严重程度。

Reduction in murine acute GVHD severity by human gingival tissue-derived mesenchymal stem cells via the CD39 pathways.

机构信息

Hepatobiliary Center, First Affiliated Hospital, Jiangsu Key Laboratory of Xenotransplantation, Collaborative Innovation Center for Cancer Medicine, Nanjing Medical University, 211166, Nanjing, Jiangsu, China.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 211166, Nanjing, Jiangsu, China.

出版信息

Cell Death Dis. 2019 Jan 8;10(1):13. doi: 10.1038/s41419-018-1273-7.

Human gingival tissue-derived mesenchymal stem cells (GMSCs) present an accessible source of mesenchymal stem cells (MSCs) for treating autoimmune diseases. Here we show that human GMSCs can prevent and treat acute graft-versus-host disease (GVHD) in two different mouse models. Our results indicate that besides exhibiting suppressive function in vitro and in vivo, GMSCs may also regulate the conversion of Tregs to Th1 and/or Th17-like cells, as well as stabilize Foxp3 expression. Furthermore, GMSC-mediated prevention of acute GVHD was dependent on CD39 signaling that play an important role in the function and stability of Tregs. Finally, we also observed stronger protective ability of GMSCs with greater expansion ability compared with BMSCs or ASCs. These results indicate that human GMSCs have the potential to be used to treat GVHD.

人牙龈组织来源的间充质干细胞（GMSCs）为治疗自身免疫性疾病提供了一种易于获取的间充质干细胞（MSCs）来源。在这里，我们证明人 GMSCs 可以在两种不同的小鼠模型中预防和治疗急性移植物抗宿主病（GVHD）。我们的结果表明，除了在体外和体内具有抑制功能外，GMSCs 还可能调节 Tregs 向 Th1 和/或 Th17 样细胞的转化，以及稳定 Foxp3 的表达。此外，GMSC 介导的急性 GVHD 的预防依赖于 CD39 信号，该信号在 Tregs 的功能和稳定性中发挥重要作用。最后，我们还观察到与 BMSCs 或 ASCs 相比，具有更强扩增能力的 GMSCs 具有更强的保护能力。这些结果表明，人 GMSCs 具有治疗 GVHD 的潜力。