Departments of Pharmacology and Pathology, Medical Faculty University of Zagreb, Zagreb 10000, Croatia.
World J Gastroenterol. 2018 Dec 28;24(48):5462-5476. doi: 10.3748/wjg.v24.i48.5462.
To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents.
Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d.
Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present).
The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels.
研究 BPC 157 和一氧化氮（NO）系统药物对穿孔盲肠病变的对抗作用。
在对穿孔盲肠损伤的大鼠应用（1 分钟后，药物 (/kg，10 mL/2 分钟浴/大鼠）包括：BPC 157（10 μg）、L-NAME（5 mg）、L-精氨酸（100mg）单独或联合，以及生理盐水浴（对照））的同时，我们连续评估了 USB 显微镜快速向盲肠表面缺损处传播的血管大体再现、缺损收缩、出血衰减、15 分钟时盲肠组织中的 MDA-和 NO 水平以及 1 天和 7 天时盲肠损伤和粘连的严重程度。
损伤后，在生理盐水浴期间/之后，血管数量明显减少，缺陷变窄，出血明显，MDA 水平升高，NO 水平降低。BPC 157 浴：血管表现明显增加，缺陷明显变窄，出血时间缩短，MDA 和 NO 水平保持正常。L-NAME：减少血管表现，但不超过对照，不改变缺陷并缩短出血。L-精氨酸：表现出较少的血管减少，不改变缺陷并延长出血。联合应用时，发生相互拮抗（L-NAME+L-精氨酸）或表现类似于 BPC 157 大鼠（BPC 157+L-NAME；BPC 157+L-精氨酸；BPC 157+L-NAME+L-精氨酸），除缺陷未改变外。因此，在第 1 天和第 7 天，生理盐水、L-NAME、L-精氨酸和 L-NAME+L-精氨酸失败（缺陷仍然开放，粘连严重）。
BPC 157 单独或与 L-NAME 和 L-精氨酸联合应用取得了治疗效果，因为它能够巩固 NO 系统对更有效愈合的刺激和抑制作用，从而招募血管。
