Department of Hygiene and Health Promotion Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima City, Kagoshima 890-8544, Japan.
Department of Life and Environmental Science, Kagoshima Prefectural College, Kagoshima City, Kagoshima 890-0005, Japan.
J Diabetes Res. 2018 Dec 3;2018:9670871. doi: 10.1155/2018/9670871. eCollection 2018.
Different involvement of leptin signaling in food intake (FI) and body temperature (BT) in pups and adults has been suggested. However, the leptin receptor (Lepr) long-form-deficient () mouse line has not been fully examined in pups. In the most available mouse line, wild-type (WT) mice have a mutation in the dedicator of cytokinesis 7 gene, named , which was recently revealed to be involved in neuronal development. Therefore, we established a line of mice without the mutation using natural mating. Adult (8 weeks of age) homozygous / mice displayed significantly higher core body weight (BW) and FI and significantly lower core BT than WT mice. However, postnatal (2 weeks of age) / mice displayed similar BW and milk intake and significantly lower core BT than WT mice. Correspondingly, adult and postnatal / mice exhibited altered mRNA levels of hypothalamic orexigenic and anorexigenic peptide in adults but not in pups. Additionally, / mice displayed significantly lower mRNA levels of brown adipose tissue uncoupling protein 1 at both ages. In conclusion, the mouse line without the mutation clearly showed the different involvements of the Lepr long form in FI and BT in pups and adults.
已有研究表明，瘦素信号在幼崽和成年动物的摄食（FI）和体温（BT）中的作用不同。然而，瘦素受体（Lepr）长型缺失（）小鼠品系在幼崽中尚未得到充分研究。在最常用的 Lepr 基因敲除小鼠模型中，野生型（WT）小鼠存在着一个细胞分裂蛋白 7 基因（）的突变，这个突变最近被揭示与神经元发育有关。因此，我们利用自然交配建立了一条没有突变的 Lepr 基因敲除小鼠品系。成年（8 周龄）杂合子 / 小鼠的核心体重（BW）、FI 显著高于 WT 小鼠，核心 BT 显著低于 WT 小鼠。然而，新生（2 周龄） / 小鼠的 BW、牛奶摄入量与 WT 小鼠相似，核心 BT 显著低于 WT 小鼠。相应地，成年和新生 / 小鼠的下丘脑食欲肽和厌食肽的 mRNA 水平在成年鼠中发生改变，但在幼鼠中没有改变。此外，/ 小鼠在两个年龄段的棕色脂肪组织解偶联蛋白 1 的 mRNA 水平均显著降低。综上所述，这条没有突变的 Lepr 基因敲除小鼠品系清楚地表明 Lepr 长型在幼崽和成年动物的 FI 和 BT 中的作用不同。
