State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, 361102 Xiamen, Fujian, China.
State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, 361102 Xiamen, Fujian, China.
Talanta. 2019 Apr 1;195:613-618. doi: 10.1016/j.talanta.2018.11.077. Epub 2018 Nov 23.
An automatic reserve flow injection method using vanadium (III) as a reducing agent for the simultaneous determination of nitrite and nitrate in estuarine and coastal waters was reported for the first time. Vanadium (III) chloride was used as an environmentally friendly substitute to toxic cadmium for reducing nitrate to nitrite. The experimental parameters were optimized based on a univariate experimental design. The salinity effect of estuarine and coastal waters was carefully investigated. Under the optimized conditions, the detection limit of the proposed method was 0.06 µmol L and 0.13 µmol L, and the linearity was up to 20 µmol L and 80 µmol L for nitrite and nitrate detection, respectively. The relative standard deviations were below 1.5% (n = 7). The recovery of spiked estuarine and coastal water samples varied from 100.0 ± 2.5% to 107.5 ± 2.5% for nitrite and 90.7 ± 0.3% to 98.0 ± 1.0% for nitrate. The sample throughput was approximately 15 h. The analytical results obtained with the proposed method displayed good agreement with the classic copperized cadmium column reduction method. The automatic reserve flow injection method has been successfully applied to analyze the nitrite and nitrate in estuarine and coastal water samples.
首次报道了一种自动储备流动注射法，使用三价钒（III）作为还原剂，同时测定河口和沿海水域中的亚硝酸盐和硝酸盐。三价氯化钒被用作替代有毒镉的环保试剂，将硝酸盐还原为亚硝酸盐。实验参数基于单因素实验设计进行了优化。仔细研究了河口和沿海水域的盐度效应。在优化条件下，该方法的检测限分别为 0.06µmol L 和 0.13µmol L，线性范围分别高达 20µmol L 和 80µmol L 用于亚硝酸盐和硝酸盐的检测。相对标准偏差低于 1.5%（n=7）。加标河口和沿海水样的回收率在亚硝酸盐为 100.0±2.5%至 107.5±2.5%之间变化，在硝酸盐为 90.7±0.3%至 98.0±1.0%之间变化。样品通量约为 15 小时。与经典的铜化镉柱还原法相比，该方法的分析结果具有良好的一致性。自动储备流动注射法已成功应用于河口和沿海水样中亚硝酸盐和硝酸盐的分析。
