INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
Talanta. 2019 Apr 1;195:699-705. doi: 10.1016/j.talanta.2018.11.070. Epub 2018 Nov 30.
This article describes the development of a new electrochemical platform composed by a polymer mixture and graphene oxide (GO). The working electrode of a screen-printed carbon electrode (SPCE) was modified with nanocomposite constituted by poly-vinyl alcohol (PVA), poly-vinylpyrrolidone (PVP) and GO, which was electrochemically reduced to obtain PVA/PVP/RGO/SPCE. The interactions and morphology of the PVA/PVP/GO nanocomposite were investigated by SEM, FTIR and UV-Vis. SEM images indicated an excellent dispersion of the GO sheets in the polymer matrix. Besides, FTIR and visible UV studies revealed strong interactions between polymer mixture and GO sheets. According to electrochemical studies, the new platform increased the electroactive surface area by a factor of 20.46 compared to the unmodified SPCE. Also, the PVA/PVP/RGO/SPCE had a fast electron kinetics transfer process with a value of k = 9.6 s. The modified electrode was applied to the determination of IgG anti-T. gondii antibodies for the serological diagnosis of toxoplasmosis. The IgG anti-T. gondii antibodies quantification showed a detection limit of 0.012 U mL, and the coefficients of variation intra-day and inter-day assays were lower than 4.5% and 6.2%, respectively. The electrochemical platform proved to be a sensitive and easily applicable tool applied to the serological diagnosis of toxoplasmosis. Therefore, the developed nanocomposite represents an excellent alternative for the electrochemical biosensor fabrication.
本文介绍了一种由聚合物混合物和氧化石墨烯 (GO) 组成的新型电化学平台的开发。通过将纳米复合材料电还原到工作电极上，对丝网印刷碳电极 (SPCE) 进行了修饰，该纳米复合材料由聚乙烯醇 (PVA)、聚乙烯吡咯烷酮 (PVP) 和 GO 组成。采用 SEM、FTIR 和 UV-Vis 研究了 PVA/PVP/GO 纳米复合材料的相互作用和形态。SEM 图像表明 GO 片在聚合物基质中具有良好的分散性。此外，FTIR 和可见 UV 研究表明聚合物混合物与 GO 片之间存在强烈的相互作用。根据电化学研究，与未修饰的 SPCE 相比，新平台的电活性表面积增加了 20.46 倍。此外，PVA/PVP/RGO/SPCE 具有快速的电子动力学转移过程，k 值为 9.6 s。修饰后的电极被用于检测 IgG 抗弓形虫抗体，用于弓形虫病的血清学诊断。IgG 抗弓形虫抗体的定量检测显示检测限为 0.012 U mL，日内和日间测定的变异系数分别低于 4.5%和 6.2%。电化学平台被证明是一种灵敏且易于应用的工具，可用于弓形虫病的血清学诊断。因此，所开发的纳米复合材料代表了用于电化学生物传感器制造的一种极好的替代方法。
