[Glutamine inhibits the inflammation in preterm rats with lung injury induced by hyperoxia and its mechanism].

Objective To investigate the effect of glutamine on the preterm hyperoxia-induced lung inflammation injury of rat models. Methods The rat model of lung injury induced by preterm hyperoxia was prepared and treated with glutamine. Diff-Quik staining was used to detect the aggregation of inflammatory cells in the bronchoalveolar lavage fluid (BALF), and HE staining was used to observe the inflammation of lung tissues. TUNEL staining was performed to detect the cell apoptosis in the lung tissues, and ELISA to test the levels of IL-1β and tumor necrosis factor α (TNF-α) in BALF. The phosphorylation of mitogen-activated protein kinase phosphatase-1 (MKP-1), mitogen-activated protein kinase (MAPK) and cytosolic phospholipase A2 (cPLA2) in the lung tissues were detected by Western blotting. Results Glutamine alleviated lung inflammation response and cell apoptosis, and reduced the levels of IL-1β and TNF-α in the inflammatory lung tissues of premature rats. Meanwhile, glutamine promoted the phosphorylation of MKP-1, and inhibited the activation of MAPK and cPLA2. Conclusion Glutamine inhibits pulmonary inflammation in preterm hyperoxia-induced lung injury rat models via regulating MKP-1/MAPK signaling pathway.