Zyuz'kov G N, Miroshnichenko L A, Udut E V, Chaikovskii A V, Polyakova T Yu, Simanina E V, Stavrova L A, Agafonov V I, Zhdanov V V
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
Bull Exp Biol Med. 2019 Jan;166(3):317-320. doi: 10.1007/s10517-019-04341-2. Epub 2019 Jan 9.
The in vitro and in vivo models of ethanol-induced neurodegeneration were used to evaluate the content and functional activity of various types of regeneration-competent cells in subventricular zone of the cerebral hemispheres in C57Bl/6JY mice. In nervous tissue culture, ethanol (65 mM) produced no effect on formation of neurospheres. When administered per os in a daily dose of 3 g/kg for 8 weeks, ethanol produced no effect on the number of neural CFU in situ. In both cases, ethanol reduced proliferative activity of neural CFU. Long-term administration of ethanol in vivo suppressed differentiation of neural stem cells and decreased the number of committed precursors (neural cluster-forming units) in the subventricular zone of cerebral hemispheres. In vitro application of ethanol stimulated secretion of humoral growth factors by the cluster-forming neural glial cells. In contrast, in vivo administration of ethanol suppressed this secretion.
利用乙醇诱导神经变性的体外和体内模型，评估C57Bl/6JY小鼠大脑半球脑室下区各种具有再生能力细胞的含量和功能活性。在神经组织培养中，乙醇（65 mM）对神经球的形成没有影响。当以每日3 g/kg的剂量口服给药8周时，乙醇对原位神经集落形成单位（CFU）的数量没有影响。在这两种情况下，乙醇均降低了神经CFU的增殖活性。在体内长期给予乙醇会抑制神经干细胞的分化，并减少大脑半球脑室下区定向前体细胞（神经簇形成单位）的数量。在体外应用乙醇可刺激形成簇的神经胶质细胞分泌体液生长因子。相反，在体内给予乙醇会抑制这种分泌。
