Peng Yu-Ke, Lu Jun-Ling, Chen Hui-Ping, Xiao Lin
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
Huan Jing Ke Xue. 2018 Nov 8;39(11):4938-4945. doi: 10.13227/j.hjkx.201801195.
In order to reveal the dynamic changes of nitrogen-transforming and phosphorus-accumulating bacteria in the process of cyanobacterial bloom formation, bacterial community structure and functional genes associated with nitrogen cycling were analyzed using high-throughput sequencing and real-time quantitative PCR (qPCR). The high-throughput sequencing results showed that the diversity of bacterial communities decreased during cyanobacterial blooms, and the lacustrine free-living and bloom-attached bacterial communities varied with increased bloom density. With increasing density of cyanobacteria, the relative abundance of Actinobacteria and Bacteroidetes decreased, whereas that of Firmicutes increased. Moreover, the growth of phosphate-accumulating organisms was increased in the phycosphere of cyanobacterial blooms, while that of nitrifiers was inhibited. Denitrifiers increased significantly under moderate blooms. The qPCR data also revealed that the abundance of nitrification and denitrification genes decreased or disappeared with the development of cyanobacterial blooms, indicating that the nitrogen transformation process might be inhibited, which showed a positive feedback of bacterial community to the development of blooms by satisfying the needs of the nutrients budget.
为揭示蓝藻水华形成过程中氮转化和磷积累细菌的动态变化，采用高通量测序和实时定量PCR（qPCR）分析了与氮循环相关的细菌群落结构和功能基因。高通量测序结果表明，蓝藻水华期间细菌群落多样性降低，湖泊中自由生活和附着于水华的细菌群落随水华密度增加而变化。随着蓝藻密度增加，放线菌和拟杆菌的相对丰度降低，而厚壁菌门的相对丰度增加。此外，在蓝藻水华的藻际环境中，聚磷菌的生长增加，而硝化细菌的生长受到抑制。在适度水华条件下，反硝化细菌显著增加。qPCR数据还表明，随着蓝藻水华的发展，硝化和反硝化基因的丰度降低或消失，这表明氮转化过程可能受到抑制，通过满足营养物质平衡的需求，细菌群落对水华发展呈现出正反馈作用。
