Fu Kun-Ming, Fu Chao, Li Hui, Jiang Shan, Qiu Fu-Guo, Cao Xiu-Qin
Sino-Dutch R & D Centre for Future Wastewater Treatment, Key Laboratory of Urban Storm water System and Water Environment, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
Huan Jing Ke Xue. 2018 Dec 8;39(12):5596-5604. doi: 10.13227/j.hjkx.201805164.
The completely autotrophic ammonium removal over nitrite(CANON)biofilm reactor acclimated by high-strength ammonia wastewater was used to treat low-strength ammonia wastewater. The treatment can be divided into three stages:① the nitrogen removal efficiency of anaerobic ammonia oxidation was low during the continuous aeration stage with inorganic wastewater as raw water (0-59 d) and with an aeration amount of 30 mL·min and ammonia concentration of 80 mg·L (until day 56), the TN removal load was only 0.13 kg·(m·d); ② during the continuous aeration stage with domestic wastewater as raw water (60-110 d), the addition of organic carbon improved the TN removal load to 0.22 kg·(m·d) on day 79; the removal rate of NH-N then reached 100% when the aeration volume improved to 100 mL·min on day 103; however, the TN removal efficiency and TN removal load decreased to 42.36% and 0.14 kg·(m·d), respectively. ③ To increase both the NH-N and TN removal efficiency during the intermittent aeration stage with domestic wastewater as raw water (110-160 d), the aeration amount was increased to 50 mL·min, while aeration was continued for 30 min and was stopped for the next 30 min; on day 131, the NH-N removal efficiency increased to 86.34%, the TN removal efficiency and removal load reached 85.87% and 0.3 kg·(m·d) respectively; on day 141, the aeration was increased to 100 mL·min and the removal efficiency of NH-N reached 100%, while the removal efficiency and removal load of TN were 64.28% and 0.22 kg·(m·d), respectively, indicating that the intermittent aeration strategy effectively improves the nitrogen removal performance of the CANON reactor. To analyze the variation of the microbial community during different stages, the samples of three stages (0, 56, and 152 d) were analyzed using high-throughput sequencing technology. The results show that:① is less affected than during the low-strength ammonia stages with inorganic and domestic wastewater as raw water; ② and were the dominant bacteria of AOB(ammonia oxidizing bacteria) and NOB (nitrite oxidizing bacteria), respectively. Domestic wastewater had a greater impact on than on ; ③ Denitrifying bacteria were present during the whole stage; and were the most adaptable, even though their relative abundances during each stage were below 0.5%.
采用经高强度氨氮废水驯化的全程自养亚硝化-厌氧氨氧化（CANON）生物膜反应器处理低强度氨氮废水。处理过程可分为三个阶段：①以无机废水为原水的连续曝气阶段（0 - 59天），曝气流量为30 mL·min，氨氮浓度为80 mg·L（至第56天），厌氧氨氧化的脱氮效率较低，总氮去除负荷仅为0.13 kg·(m³·d)；②以生活污水为原水的连续曝气阶段（60 - 110天），添加有机碳后，第79天总氮去除负荷提高至0.22 kg·(m³·d)；第103天曝气流量提高至100 mL·min时，氨氮去除率达到100%；然而，总氮去除效率和总氮去除负荷分别降至42.36%和0.14 kg·(m³·d)。③以生活污水为原水的间歇曝气阶段（110 - 160天），为提高氨氮和总氮去除效率，曝气流量增加至50 mL·min，曝气30 min，停曝30 min；第131天，氨氮去除效率提高至86.34%，总氮去除效率和去除负荷分别达到85.87%和0.3 kg·(m³·d)；第141天，曝气流量增加至100 mL·min，氨氮去除效率达到100%，总氮去除效率和去除负荷分别为64.28%和0.22 kg·(m³·d)，表明间歇曝气策略有效提高了CANON反应器的脱氮性能。为分析不同阶段微生物群落的变化，采用高通量测序技术对三个阶段（0、56和152天）的样品进行了分析。结果表明：①在以无机废水和生活污水为原水的低强度氨氮阶段， 受影响程度小于 ；② 和 分别是氨氧化细菌（AOB）和亚硝酸盐氧化细菌（NOB）的优势菌属。生活污水对 的影响大于对 的影响；③整个阶段均存在反硝化细菌； 和 适应性最强，尽管它们在各阶段的相对丰度均低于0.5%。
