Jin Sil, O Jiyun, Stellabotte Frank, Brown Susan J, Choe Chong Pyo
Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 52828, South Korea.
Division of Life Science, Gyeongsang National University, Jinju, 52828, South Korea.
Gene Expr Patterns. 2019 Jan;31:26-31. doi: 10.1016/j.gep.2019.01.002. Epub 2019 Jan 7.
The pair-rule gene teneurin-m/odd Oz (ten-m/odz) is required for the patterning of alternate segment boundaries in the early Drosophila embryo. Mutant phenotypes of ten-m/odz display a typical pair-rule phenotype in which odd-numbered segments are eliminated. Consistent with its pair-rule function, Ten-m/Odz protein is expressed in a seven-stripe pattern before the onset of gastrulation. While expression of ten-m/odz orthologues have been characterized in several vertebrate species, their expression patterns in non-Drosophila arthropods during embryonic segmentation have yet to be reported. Here, we have identified a Tribolium orthologue of ten-m/odz (Tc-ten-m/odz) and analyzed its expression patterns during embryonic segmentation. Tc-ten-m/odz expression was observed in a region of the growth zone, which appeared to be a potential mesodermal region, during germband elongation. Later, segmental expression appeared in the trunk after segments had already formed. In contrast to Drosophila, apparently Tc-ten-m/odz was neither expressed in the ectoderm of the growth zone where segmentation occurs, nor the ectoderm of trunk regions where segmentation is maintained. Our findings suggest that Tc-ten-m/odz may not function as a pair rule gene in Tribolium segmentation.
配对规则基因teneurin-m/odd Oz（ten-m/odz）在果蝇早期胚胎中交替节段边界的模式形成中是必需的。ten-m/odz的突变表型显示出典型的配对规则表型，其中奇数节段被消除。与其配对规则功能一致，Ten-m/Odz蛋白在原肠胚形成开始前以七条带的模式表达。虽然ten-m/odz直系同源物在几种脊椎动物物种中的表达已得到表征，但它们在非果蝇节肢动物胚胎分割过程中的表达模式尚未见报道。在这里，我们鉴定了ten-m/odz的赤拟谷盗直系同源物（Tc-ten-m/odz），并分析了其在胚胎分割过程中的表达模式。在胚带伸长期间，在生长区的一个区域观察到Tc-ten-m/odz表达，该区域似乎是一个潜在的中胚层区域。后来，在节段已经形成后，节段性表达出现在躯干中。与果蝇不同，显然Tc-ten-m/odz既不在发生分割的生长区外胚层中表达，也不在维持分割的躯干区域外胚层中表达。我们的研究结果表明，Tc-ten-m/odz在赤拟谷盗分割中可能不发挥配对规则基因的作用。
