Biomed Mater. 2019 Feb 8;14(2):025012. doi: 10.1088/1748-605X/aafd7c.
Glutaraldehyde (GLUT) crosslinked bioprosthetic heart valves (BHVs) might fail within ten years due to progressive degradation and calcification. GLUT cannot stabilize glycosaminoglycans (GAGs), one important component of BHVs. In this current study we developed a new BHVs preparation strategy named as 'HPA/NT/HRP' treatment that utilized 3,4-hydroxyphenylpropionic acid (HPA)/tyramine (TRA)/neomycin trisulfate (NT) conjugated pericardiums and horseradish peroxidase (HRP)/HO enzyme-oxidative-polymerization method. HPA/TRA-pericardium and HPA-NT conjugation would provide extra phenol groups for enzymatic crosslinking. HPA/TRA conjugated pericardium could be crosslinked by HRP/HO enzyme-oxidative-polymerization. The feeding ratio of HPA-NT was optimized. The GAGs content, collagenase and elastase degradation in vitro, the in vivo GAGs stability and anti-calcification potential of HPA/NT/HRP treated pericardiums were characterized. We demonstrated that HPA/NT/HRP treated pericardiums had sufficiently increased GAGs stabilization and decreased calcification. This new HPA/NT/HRP enzyme-oxidative-polymerization strategy would be a promising method to make BHVs with better GAGs stability and anti-calcification properties.
戊二醛（GLUT）交联生物心脏瓣膜（BHVs）可能在十年内失效，原因是其渐进性降解和钙化。GLUT 不能稳定糖胺聚糖（GAGs），这是 BHVs 的一个重要组成部分。在本研究中，我们开发了一种新的 BHVs 制备策略，称为“HPA/NT/HRP”处理，该策略利用 3,4-羟基苯丙酸（HPA）/酪胺（TRA）/新霉素三硫酸盐（NT）共轭心包和辣根过氧化物酶（HRP）/HO 酶氧化聚合方法。HPA/TRA 心包和 HPA-NT 缀合将提供额外的酚基用于酶交联。HPA/TRA 共轭心包可通过 HRP/HO 酶氧化聚合进行交联。优化了 HPA-NT 的进料比。研究了 HPA/NT/HRP 处理的心包膜的 GAGs 含量、体外胶原酶和弹性蛋白酶降解、体内 GAGs 稳定性和抗钙化潜力。结果表明，HPA/NT/HRP 处理的心包膜具有足够高的 GAGs 稳定性和降低的钙化。这种新的 HPA/NT/HRP 酶氧化聚合策略将成为制造具有更好 GAGs 稳定性和抗钙化性能的 BHVs 的有前途的方法。
