Bäumer F, Hartmann A A, Henrich H A
Chirurgischen Klinik und Poliklinik, Universität Würzburg.
Zentralbl Chir. 1988;113(12):793-800.
Experimental studies were conducted into the biological durability of homologous full skin grafts. Colony counts for that purpose were determined in deep-frozen specimens in weekly intervals. Specimens were stored at various temperatures (+4 degrees C, -20 degrees C, -70 degrees C, -80 degrees C), with and without preceding quick freezing. Storage at -70 degrees C with protracted freezing gradient of 1 degree C/minute proved to the most favourable variant with due consideration of microbiological criteria. The physiological resident flora of skin grafts after thawing was almost unchanged under these conditions.
对同种异体全厚皮片的生物耐久性进行了实验研究。为此目的，对深度冷冻的标本每周进行一次菌落计数。标本在不同温度（+4℃、-20℃、-70℃、-80℃）下储存，有无预先快速冷冻。考虑到微生物学标准，在-70℃下以1℃/分钟的缓慢冷冻梯度储存被证明是最有利的变体。在这些条件下，解冻后皮肤移植物的生理性常驻菌群几乎没有变化。
