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[全层皮肤储存的实验微生物学标准]

[Experimental microbiological criteria for the banking of full-thickness skin].

作者信息

Bäumer F, Hartmann A A, Henrich H A

机构信息

Chirurgischen Klinik und Poliklinik, Universität Würzburg.

出版信息

Zentralbl Chir. 1988;113(12):793-800.

PMID:3063019
Abstract

Experimental studies were conducted into the biological durability of homologous full skin grafts. Colony counts for that purpose were determined in deep-frozen specimens in weekly intervals. Specimens were stored at various temperatures (+4 degrees C, -20 degrees C, -70 degrees C, -80 degrees C), with and without preceding quick freezing. Storage at -70 degrees C with protracted freezing gradient of 1 degree C/minute proved to the most favourable variant with due consideration of microbiological criteria. The physiological resident flora of skin grafts after thawing was almost unchanged under these conditions.

摘要

对同种异体全厚皮片的生物耐久性进行了实验研究。为此目的,对深度冷冻的标本每周进行一次菌落计数。标本在不同温度(+4℃、-20℃、-70℃、-80℃)下储存,有无预先快速冷冻。考虑到微生物学标准,在-70℃下以1℃/分钟的缓慢冷冻梯度储存被证明是最有利的变体。在这些条件下,解冻后皮肤移植物的生理性常驻菌群几乎没有变化。

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