一种分析根向水性反应的快速分析方法。

A method for rapid analysis of the root hydrotropic response in .

机构信息

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor., 62210, México.

出版信息

Biotechniques. 2019 Mar;66(3):154-158. doi: 10.2144/btn-2018-0173. Epub 2019 Jan 11.

DOI:10.2144/btn-2018-0173

PMID:30630346

Abstract

The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of KCO with a density of 1.48 g·ml to generate a rapid moisture gradient inside a square Petri dish without decreasing root growth. In this, the root growth rate observed in hydrostimulated wild-type and mutant, utilized as a negative control, increases sixfold compared with those roots examined using the former method.

摘要

分析野生型根中具有湿度梯度的向水曲率的系统得到了改进。确定了在刺激后 4 小时检测 90°向水曲率的最佳条件。该系统仅需要 15ml 密度为 1.48g/ml 的 KCO 溶液，即可在不减少根生长的情况下在方形培养皿内产生快速的湿度梯度。在此，用作负对照的水刺激野生型和突变体中观察到的根生长速率与使用前一种方法检查的根相比增加了六倍。